The Oral Microbiome of Healthy Japanese People at the Age of 90
The Oral Microbiome of Healthy Japanese People at the Age of 90
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DOI：
10.3390/app10186450
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发表时间：
2020-09
期刊：
影响因子：
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通讯作者：
Y. Nomura;E. Kakuta;N. Kaneko;K. Nohno;A. Yoshihara;N. Hanada
中科院分区：
文献类型：
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作者：
Y. Nomura;E. Kakuta;N. Kaneko;K. Nohno;A. Yoshihara;N. Hanada
对于健康的口腔，保持健康的微生物组是必不可少的。然而，关于健康微生物组的数据并不充分。为了确定核心微生物组的性质，使用焦磷酸测序数据分析口腔微生物组结构。唾液样本来自参加20年随访新泻队列研究的90岁健康参与者。共有85人参加了健康检查。研究人群包括40名男性和45名女性参与者。通过咀嚼石蜡5 min获得刺激唾液样本。通过PCR扩增16 S核糖体RNA（rRNA）基因的V3-V4高变区。使用MiSeq进行焦磷酸测序。基于EzTaxon-e数据库中的97%同一性检索分配操作分类单位（OTU）。在物种水平上使用100%检测阈值，检测到13种物种：中华链球菌、肺炎链球菌、唾液链球菌、KV 831974_s、副血链球菌、Veillonella dispar、Granulicatella adiacens、Streptococcus_uc、链球菌、KE952139_s、小韦荣球菌、小变形菌，这些物种代表了人类微生物组核心组成的潜在候选者。
For a healthy oral cavity, maintaining a healthy microbiome is essential. However, data on healthy microbiomes are not sufficient. To determine the nature of the core microbiome, the oral-microbiome structure was analyzed using pyrosequencing data. Saliva samples were obtained from healthy 90-year-old participants who attended the 20-year follow-up Niigata cohort study. A total of 85 people participated in the health checkups. The study population consisted of 40 male and 45 female participants. Stimulated saliva samples were obtained by chewing paraffin wax for 5 min. The V3–V4 hypervariable regions of the 16S ribosomal RNA (rRNA) gene were amplified by PCR. Pyrosequencing was performed using MiSeq. Operational taxonomic units (OTUs) were assigned on the basis of a 97% identity search in the EzTaxon-e database. Using the threshold of 100% detection on the species level, 13 species were detected: Streptococcus sinensis, Streptococcus pneumoniae, Streptococcus salivarius, KV831974_s, Streptococcus parasanguinis, Veillonella dispar, Granulicatella adiacens, Streptococcus_uc, Streptococcus peroris, KE952139_s, Veillonella parvula, Atopobium parvulum, and AFQU_vs. These species represent potential candidates for the core make-up of the human microbiome.