Structural characterization of neutral and anionic glucans from Mesorhizobium loti
Structural characterization of neutral and anionic glucans from Mesorhizobium loti
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DOI：
10.1016/j.carres.2008.07.007
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发表时间：
2008-09-22
影响因子：
3.1
通讯作者：
Mitsui, Hisayuki
中科院分区：
文献类型：
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作者：
Kawaharada, Yasuyuki;Kiyota, Hiromasa;Mitsui, Hisayuki
对中生根瘤菌（Mesorhizobium loti）的周质葡聚糖进行了分离，并根据其酸性将其分成级分。核磁共振光谱证实其主链结构是环状β-（1->2）-D-葡聚糖，与其他根瘤菌一样，并显示中性部分中没有非糖苷取代基，而甘油磷酰基和琥珀酰基残基分别是阴离子部分中的主要和次要取代基。MALDI-TOF质谱表明，阴离子葡聚糖含有一个，两个或三个这样的取代基，每个分子根据其酸度，并且，相反，所有的阴离子亚组分具有类似的大小分布的中性葡聚糖，其中分子组成的20-24个葡糖基残基是占主导地位的。这些结果阐明了M中周质葡聚糖组成的荷质比。Loti细胞。(C)2008爱思唯尔有限公司保留所有权利。
The periplasmic glucans of Mesorhizobium loti were isolated and separated into fractions according to their acidity. NMR spectroscopy confirmed their backbone structure to be a cyclic beta-(1-->2)-D-glucan as in the case of other rhizobia, and revealed no non-glycosidic substituents in the neutral fraction, and glycerophosphoryl and succinyl residues as major and minor substituents, respectively, in the anionic fractions. MALDI-TOF mass spectrometry showed that the anionic glucans contain one, two, or three such substituents per molecule according to their acidity, and, in contrast, that all the anionic subfractions have a similar size distribution to that of the neutral glucans, where molecules composed of 20-24 glucosyl residues are predominant. These results clarify the periplasmic glucan composition in terms of charge-to-mass ratios in M. loti cells. (C) 2008 Elsevier Ltd. All rights reserved.