Complete Freund's adjuvant immunization prolongs survival in experimental prion disease in mice
Complete Freund's adjuvant immunization prolongs survival in experimental prion disease in mice
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DOI：
10.1002/jnr.10474
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发表时间：
2003-01-15
影响因子：
4.2
通讯作者：
Mor, F
中科院分区：
文献类型：
--
作者：
Tal, Y;Souan, L;Mor, F
我们最近报道，免疫小鼠与某些自身朊蛋白肽诱导特异性T细胞和B细胞免疫反应，重要的是，这种免疫与减少蛋白酶抗性PrPSc颗粒可回收的移植，羊瘙痒病感染的同源肿瘤。本研究旨在确定免疫原性PrP肽是否可能影响小鼠实验性瘙痒症的自然病程。我们用自身朊病毒肽在完全弗氏佐剂（CFA）中免疫C57 BL/6小鼠或用单独的CFA作为对照，然后通过腹膜内或脑内注射感染小鼠适应性瘙痒症。我们在这里报告说，CFA免疫接种，无论是否朊病毒肽存在于接种物中，导致小鼠的生存期显着延长，无论是脑内或腹膜内的挑战。免疫组和对照组中死亡的小鼠含有等量的PrPSc。因此，CFA免疫对小鼠实验性瘙痒症具有治疗作用，可能是通过降低PrPSc在脑中的积累速率。(C)2002 Wiley-Liss，Inc.
We recently reported that immunization of mice with certain self-prion protein peptides induced specific T-cell and B-cell immune responses; importantly, this immunization was associated with a decrease in the number of protease-resistant PrPSc particles recoverable in a transplanted, scrapie-infected syngeneic tumor. The present study was carried out to determine whether immunization with the immunogenic PrP peptides might influence the natural history of experimental scrapie in mice. We immunized C57BL/6 mice with self-prion peptides in complete Freund's adjuvant (CFA) or with CFA alone as a control and then infected the mice with mouse-adapted scrapie by injection either intraperitoneally or intracerebrally. We report here that immunization with CFA, irrespective of whether prion peptides were present in the inoculum, resulted in marked prolongation of survival of the mice, whether the challenge was intracerebral or intraperitoneal. Mice in the immunized and control groups that died contained equivalent amounts of PrPSc. Thus, CFA immunization has a therapeutic effect in experimental scrapie in mice, possibly by reducing the rate of PrPSc accumulation in the brain. (C) 2002 Wiley-Liss, Inc.