Flexible and stretchable opto-electric neural interface for low-noise electrocorticogram recordings and neuromodulation in vivo
Flexible and stretchable opto-electric neural interface for low-noise electrocorticogram recordings and neuromodulation in vivo
复制标题
DOI：
10.1016/j.bios.2020.112009
复制
发表时间：
2020-04-01
影响因子：
12.6
通讯作者：
Liu, Jingquan
中科院分区：
文献类型：
--
作者：
Ji, Bowen;Ge, Chaofan;Liu, Jingquan
基于光遗传学的神经调节工具正在发展为结合光学操纵和电生理记录的动物基础神经科学研究。然而，目前贴附在大脑皮质上的皮质电图（ECoG）光电集成装置由于机械不匹配和大脑变形，仍然存在对脑组织和电极造成潜在损伤的风险。在这里，我们提出了一种可伸缩的光电集成神经接口，通过集成蛇形电极和多位微发光二极管到超弹性基板上，以及嵌入记录电极的蛇形金属屏蔽低噪声信号采集。该器件采用精细的结构设计、超软封装和先独立制作后组装的工艺，有利于提高器件的一致性、可靠性和成品率。体外加速老化试验和往复拉伸试验表明，其性能良好，稳定性高。表达视紫红质-2的清醒小鼠的局灶性皮质区的体内光遗传学激活通过同时高质量记录来实现。我们强调这种多功能神经接口的神经应用的潜在用途。
Optogenetic-based neuromodulation tools is evolving for the basic neuroscience research in animals combining optical manipulation and electrophysiological recordings. However, current opto-electric integrated devices attaching on cerebral cortex for electrocorticogram (ECoG) still exist potential damage risks for both brain tissue and electrode, due to the mechanical mismatch and brain deformation. Here, we propose a stretchable optoelectric integrated neural interface by integrating serpentine-shaped electrodes and multisite micro-LEDs onto a hyperelastic substrate, as well as a serpentine-shaped metal shielding embedded in recording electrode for low-noise signal acquisition. The delicate structure design, ultrasoft encapsulation and independent fabrication followed by assembly are beneficial to the conformality, reliability and yield. In vitro accelerated deterioration and reciprocating tensile have demonstrated good performance and high stability. In vivo optogenetic activation of focal cortical areas of awaked mouse expressing Channelrhodopsin-2 is realized with simultaneous high-quality recording. We highlight the potential use of this multifunctional neural interface for neural applications.