Enhanced photo-stability, thermal-stability and aqueous-stability of indocyanine green in polymeric nanoparticulate systems
Enhanced photo-stability, thermal-stability and aqueous-stability of indocyanine green in polymeric nanoparticulate systems
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DOI：
10.1016/j.jphotobiol.2004.01.002
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发表时间：
2004-03-19
影响因子：
5.4
通讯作者：
Shao, J
中科院分区：
文献类型：
--
作者：
Saxena, V;Sadoqi, M;Shao, J
吲哚青绿(ICG)的光降解、热降解和水的不稳定性限制了其作为成像荧光造影剂的应用。因此，本研究的目的是开发包埋ICG的聚合物纳米颗粒，并确定其在为ICG提供光稳定性、热稳定性和水稳定性方面的有效性。设计并表征了包埋ICG的纳米粒子，研究了ICG在水介质中的降解动力学。ICG被包埋在纳米粒子中，导致其峰值荧光波长发生移动，峰值荧光强度降低。在所研究的时间段内，ICG在纳米水悬浮液中的降解遵循一级动力学。包埋在纳米粒中的ICG提高了ICG的水稳定性(ICG在纳米粒中的半衰期t(1/2)为72.2±6.1h，而游离ICG溶液的半衰期为16.8±1.5h)，ICG的光稳定性和热稳定性(42℃时ICG的t(1/2)为62.4+/-1.7 h，而游离ICG溶液为10.1+/-0.6 h)。(C)2004爱思唯尔B.V.保留所有权利。
Photo-degradation, thermal-degradation and aqueous-instability of indocyanine green (ICG) limits its application as a fluorescence contrast agent for imaging purposes. Thus, the objective of this study is to develop polymeric nanoparticles entrapping ICG and to establish its effectiveness in providing photo-stability, thermal stability and aqueous stability to ICG. Nanoparticles entrapping ICG were engineered, characterized and the degradation kinetics of ICG in the nanoparticles was investigated in aqueous media. The entrapment of ICG in the nanoparticles causes a shift in its wavelength of peak fluorescence and a decrease in its peak fluorescence intensity. The degradation of ICG in aqueous nanoparticle suspension followed first-order kinetics for the time period studied. ICG entrapment in the nanoparticles enhanced aqueous-stability of ICG (half-life, t(1/2) was 72.2 +/- 6.1 h for ICG in the nanoparticles as compared to 16.8 +/- 1.5 h for free ICG solution), photo-stability of ICG (t(1/2) was 73.7 +/- 7.5 h for ICG in the nanoparticles as compared to 14.4 +/- 2.4 h for free ICG solution when exposed to room light from two 32 W normal fluorescent tubes) and thermal-stability of ICG (t(1/2) of ICG at 42degreesC was 62.4 +/- 1.7 h for ICG in the nanoparticles as compared to 10.1 +/- 0.6 h for free ICG solution). (C) 2004 Elsevier B.V. All rights reserved.