High pyrazine production by Bacillus subtilis in solid substrate fermentation on ground soybeans
High pyrazine production by Bacillus subtilis in solid substrate fermentation on ground soybeans
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DOI：
10.1016/s0032-9592(98)00141-1
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发表时间：
1999-09-01
影响因子：
4.4
通讯作者：
Gros, JB
中科院分区：
文献类型：
--
作者：
Larroche, C;Besson, I;Gros, JB
2,5-二甲基吡嗪（2,5- dmp）和四甲基吡嗪（TTMP）是由枯草芽孢杆菌IFO 3013在固体底物条件下培养而成的。优化研究表明，产生上述两种香气化合物的最佳方法是在培养基中大量富集l -苏氨酸和乙托因，氨基酸允许2,5- dmp的形成，在工艺开始时以40 g/l的浓度添加，而乙托因是TTMP的前体，只有在2,5- dmp生产结束后才能以60 g/l的浓度添加。最佳培养温度为40℃，pH监测为7.5，曝气速率大于0.1 VVM，体积氧传递系数k(L)接近180 h（-1）。在这些条件下，总吡嗪的回收率为2 g/l，证明了该方法的有效性。1999爱思唯尔科学有限公司版权所有。
2,5-Dimethylpyrazine (2,5-DMP) and tetramethylpyrazine (TTMP) were produced using Bacillus subtilis IFO 3013 grown in solid substrate conditions using ground soybeans suspended in water. Optimization studies showed that the best way to produce the two above aroma compounds involved massive enrichment of the medium with L-threonine and acetoin, The amino acid allowed 2,5-DMP formation and was added at 40 g/l at the beginning of a process, while acetoin was the precursor of TTMP and had to be added at 60 g/l only after 2,5-DMP production was terminated. The optimal cultivation temperature was 40 degrees C, pH had to be monitored at 7.5, and aeration rate had to be higher than 0.1 VVM with a volu metric oxygen tranfer coefficient k(L)a close to 180 h(-1) . These conditions allowed recovery of 2 g/l total pyrazines, that demonstra ted the efficiency of this approach. (C) 1999 Elsevier Science Ltd. All righ ts reserved.