Anesthetic preconditioning inhibits isoflurane-mediated apoptosis in the developing rat brain.
Anesthetic preconditioning inhibits isoflurane-mediated apoptosis in the developing rat brain.
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DOI：
10.1213/ane.0000000000000380
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发表时间：
2014-10
影响因子：
5.7
通讯作者：
Wei H
中科院分区：
文献类型：
--
作者：
Peng J;Drobish JK;Liang G;Wu Z;Liu C;Joseph DJ;Abdou H;Eckenhoff MF;Wei H
我们假设，预处理与短期暴露于异氟烷（ISO）将减少新生大鼠长期暴露于ISO诱导的神经变性，如先前在神经元细胞培养中所示。将7日龄SD大鼠随机分为3组：对照组、1.5%ISO组和预处理（PC）+1.5%ISO组。对照组暴露于载气（氮气中平衡30%氧气）30 min，然后第二天再次暴露于载气6 h。1.5%ISO组暴露于载气30 min，然后在第二天暴露于1.5%ISO 6 h。PC +1.5%ISO组用30 min 1.5%ISO暴露预处理，然后在第二天暴露于1.5%ISO 6 h。在暴露后2小时收集血液和脑样品以测定神经变性生物标志物，包括半胱天冬酶-3、S100 β、半胱天冬酶-12和自噬生物标志物Beclin-1。长期暴露于ISO显着增加裂解caspase-3的表达，在大脑皮层的7天大的大鼠相比，与ISO和对照组使用Western印迹分析预处理。然而，其他神经元损伤标志物未检测到显著差异。ISO介导的增加裂解caspase-3在出生后第7天的大鼠大脑的改善预处理与短暂的麻醉剂暴露，并没有检测到其他标志物的神经元损伤的差异。
We hypothesized that preconditioning with a short exposure to isoflurane (ISO) would reduce neurodegeneration induced by prolonged exposure to ISO in neonatal rats, as previously shown in neuronal cell culture. We randomly divided 7-day-old Sprague-Dawley rats into 3 groups: control, 1.5% ISO, and preconditioning (PC) + 1.5% ISO. The control group was exposed to carrier gas (30% oxygen balanced in nitrogen) for 30 min and then carrier gas again for 6 h the following day. The 1.5% ISO group was exposed to carrier gas for 30 min and then 1.5% ISO for 6 h the following day. The PC + 1.5% ISO group was preconditioned with a 30 min 1.5% ISO exposure and then exposed to 1.5% ISO for 6 h the following day. Blood and brain samples were collected 2 h after the exposures for determination of neurodegenerative biomarkers, including caspase-3, S100β, caspase-12, and an autophagy biomarker Beclin-1. Prolonged exposure to ISO significantly increased cleaved caspase-3 expression in the cerebral cortex of 7-day old rats compared to the group preconditioned with ISO and the controls using Western blot assays. However, significant differences were not detected for other markers of neuronal injury. The ISO-mediated increase in cleaved caspase-3 in the postnatal day 7 rat brain is ameliorated by preconditioning with a brief anesthetic exposure, and differences were not detected in other markers of neuronal injury.