Mobile Genetic Elements Drive Antimicrobial Resistance Gene Spread in Pasteurellaceae Species.
Mobile Genetic Elements Drive Antimicrobial Resistance Gene Spread in Pasteurellaceae Species.
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DOI：
10.3389/fmicb.2021.773284
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发表时间：
2021
影响因子：
5.2
通讯作者：
Bazzolli DMS
中科院分区：
文献类型：
--
作者：
da Silva GC;Gonçalves OS;Rosa JN;França KC;Bossé JT;Santana MF;Langford PR;Bazzolli DMS
移动遗传元件(MGES)和抗菌素耐药性(AMR)在微生物群落和病原菌与宿主之间的相互作用中驱动着重要的生态关系。在这项研究中，我们研究了345个公开可用的巴氏杆菌科基因组中的耐药组相关运动组，这是一个革兰氏阴性细菌大家族，包括主要的人类和动物病原体。我们生成了整合到基因组中的移动体的全面数据集，包括10,820个插入序列，2,939个前驱体，以及43个整合和接合元件。此外，我们还评估了巴氏杆菌科的质粒序列。我们的发现极大地扩展了该家族MGES的多样性，包括对新元素的描述。我们发现，MGES具有可比性，并分散在不同物种中，它们也共同存在于基因组中，通过基因转移对该家族的生态做出了贡献。此外，我们还研究了这些元件在AMR基因传播和形成中的影响。共有55个不同的AMR基因被映射到数据集中的721个位置。在已发现的AMR基因中，MGES与77.6%的AMR基因连锁，表明它们参与了此类基因的获取和传递。这项研究展示了与MGES相关的抗性基因的全球分布，为巴氏杆菌科提供了一个未知的视角。
Mobile genetic elements (MGEs) and antimicrobial resistance (AMR) drive important ecological relationships in microbial communities and pathogen-host interaction. In this study, we investigated the resistome-associated mobilome in 345 publicly available Pasteurellaceae genomes, a large family of Gram-negative bacteria including major human and animal pathogens. We generated a comprehensive dataset of the mobilome integrated into genomes, including 10,820 insertion sequences, 2,939 prophages, and 43 integrative and conjugative elements. Also, we assessed plasmid sequences of Pasteurellaceae. Our findings greatly expand the diversity of MGEs for the family, including a description of novel elements. We discovered that MGEs are comparable and dispersed across species and that they also co-occur in genomes, contributing to the family’s ecology via gene transfer. In addition, we investigated the impact of these elements in the dissemination and shaping of AMR genes. A total of 55 different AMR genes were mapped to 721 locations in the dataset. MGEs are linked with 77.6% of AMR genes discovered, indicating their important involvement in the acquisition and transmission of such genes. This study provides an uncharted view of the Pasteurellaceae by demonstrating the global distribution of resistance genes linked with MGEs.