Rhesus papillomavirus type 1 cooperates with activated ras in transforming primary epithelial rat cells independent of dexamethasone.
Rhesus papillomavirus type 1 cooperates with activated ras in transforming primary epithelial rat cells independent of dexamethasone.
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恒河猴乳头瘤病毒 1 型与激活的 ras 协同作用，不依赖地塞米松即可转化大鼠原代上皮细胞。
DOI：
10.1128/jvi.65.6.3354-3358.1991
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发表时间：
1991
影响因子：
5.4
通讯作者：
Faras,AJ
中科院分区：
文献类型：
--
作者：
Schneider,JF;McGlennen,RC;LaBresh,KV;Ostrow,RS;Faras,AJ
恒河猴乳头瘤病毒1型（RhPV-1）是最近从恒河猴阴茎鳞状细胞癌淋巴结转移中克隆到的。在本文中，我们证明了RhPV-1与激活的ras癌基因合作，以相当于人乳头瘤病毒16型的水平转化原代细胞。克隆病毒DNA，使得它们的表达在它们的天然启动子元件的控制下。与人乳头瘤病毒16型不同，RhPV-1 DNA与ras合作，不依赖于激素地塞米松。然而，地塞米松确实对一些RhPV-1共转化细胞在软琼脂试验中生长的能力产生了积极的影响。转化细胞在裸鼠体内具有高度致瘤性。
Rhesus Papillomavirus type 1 (RhPV-1) was recently cloned from a rhesus monkey lymph node metastasis of a penile squamous cell carcinoma. In this paper, we demonstrate that RhPV-1 cooperates with the activated ras oncogene to transform primary cells at a level comparable to human papillomavirus type 16. The viral DNAs were cloned such that their expression was under the control of their natural promoter elements. Unlike human papillomavirus type 16, RhPV-1 DNA cooperated with ras independently of the hormone dexamethasone. However, dexamethasone did have a positive influence on the ability of some RhPV-1 cotransformed cells to grow in soft-agar assays. The transformed cells are highly tumorigenic in vivo in nude mice.