Effect of nucleotides supplementation to low fish meal feed on long-chain polyunsaturated fatty acids composition of juvenile rainbow trout Oncorhynchus mykiss
Effect of nucleotides supplementation to low fish meal feed on long-chain polyunsaturated fatty acids composition of juvenile rainbow trout Oncorhynchus mykiss
复制标题
低鱼粉饲料中添加核苷酸对虹鳟幼鱼长链多不饱和脂肪酸组成的影响
DOI：
10.1111/are.14103
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发表时间：
2019
影响因子：
2
通讯作者：
S.Satoh
中科院分区：
文献类型：
--
作者：
A.Rudwanudin;Y.Haga;S.Satoh
进行饲养试验以评估在低鱼粉饲料中添加核苷酸对虹鳟鱼的生长和脂肪酸组成的影响。六等氮（42%粗蛋白）和等脂（18%粗脂肪）的饮食配方含有鱼粉和植物成分作为主要蛋白质来源。对照日粮为不添加核苷酸的基础日粮，通过在基础日粮中添加5种不同核苷酸中的一种（0.15%），即肌苷（IMP）、腺苷（AMP）、鸟苷（GMP）、尿苷（UMP）和胞苷（CMP），制备5种实验日粮。将240尾初始平均体重为9.8g的虹鳟幼鱼随机分配到12个水族箱中。经过15周的饲养期，虹鳟鱼的生长性能和饲料利用率没有显着差异的饲料处理。饲料GMP、UMP和CMP易于在肌肉和鱼体中积累粗脂肪。此外，饲料GMP，UMP和CMP显着增加肝脏18：3 n-3和长链同系物18：4 n-3和20：4 n-3含量。肝脏18：2n-6含量也显示在喂食GMP、UMP和CMP饲料的鱼中增加，但长链同系物20：3 n-6和20：4 n-6含量降低。在IMP、GMP和CMP饲料中，肌肉中20：4 n-6、20：5 n-3和22：6 n-3含量也有所下降。本研究清楚地表明，饲料核苷酸对鱼的生长没有积极的影响，但饲料核苷酸，特别是GMP，UMP和CMP改变虹鳟鱼的多不饱和脂肪酸组成。
A feeding trial was conducted to evaluate the effect of nucleotides supplementation to low‐fish meal feed on growth and fatty acid composition of rainbow trout. Six isonitrogenous (42% crude protein) and isolipidic (18% crude lipid) diets were formulated containing fish meal and plant ingredients as main protein sources. The control diet was a basal diet without supplementation of nucleotides, and five experimental diets were prepared by supplementing one of the five different nucleotides in the form of 5′‐monophosphate (0.15%), that is inosine (IMP), adenosine (AMP), guanosine (GMP), uridine (UMP) and cytidine (CMP) onto basal diet. Two hundred forty juvenile rainbow trout with an initial average body weight 9.8 g were randomly distributed into twelve aquaria. After 15 weeks of feeding period, growth performance and feed utilization of rainbow trout were not significantly different among dietary treatments. Dietary GMP, UMP and CMP tended to accumulate crude lipid in the muscle and whole fish body. Moreover, dietary GMP, UMP and CMP significantly increased hepatic 18:3n‐3 and long‐chain homologue 18:4n‐3 and 20:4n‐3 contents. Hepatic 18:2n‐6 content showed also increase in fish fed GMP, UMP and CMP diets, but decreased in long‐chain homologue 20:3n‐6 and 20:4n‐6 contents. Decrease in 20:4n‐6, 20:5n‐3 and 22:6n‐3 contents was also found in the muscle of fish fed IMP, GMP and CMP diets. The present study clearly showed that there was no positive effect of dietary nucleotides on growth of fish, but dietary nucleotides particularly GMP, UMP and CMP altered polyunsaturated fatty acid composition of rainbow trout.