Recapitulation of developmental cardiogenesis governs the morphological and functional regeneration of adult newt hearts following injury
Recapitulation of developmental cardiogenesis governs the morphological and functional regeneration of adult newt hearts following injury
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DOI：
10.1016/j.ydbio.2011.03.021
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发表时间：
2011-06-01
影响因子：
2.7
通讯作者：
Morrison, Jamie Ian
中科院分区：
文献类型：
--
作者：
Witman, Nevin;Murtuza, Bari;Morrison, Jamie Ian
有尾目两栖动物，如蝾螈，是“再生冠军”，因为它们能够再生许多身体部位和组织。然而，之前的实验表明，蝾螈心脏的再生能力有限，与人类心脏相似。使用一种新颖的、可重复的心室切除模型，我们首次证明成年蝾螈心脏可以完全再生，没有任何疤痕证据。这一过程是由心脏发育过程中通常表达的心脏转录因子的增殖增加和上调控制的。此外，我们能够识别心肌新再生区域内表达 LIM 同源结构域蛋白 Istet1 和 GATA4（心脏祖细胞中发现的转录因子）的细胞。使用蝾螈作为模式生物获得的信息可能有助于揭示受损人类心脏的再生缺陷。 (c) 2011 Elsevier Inc. 保留所有权利。
Urodele amphibians, like the newt, are the "champions of regeneration" as they are able to regenerate many body parts and tissues. Previous experiments, however, have suggested that the newt heart has only a limited regeneration capacity, similar to the human heart. Using a novel, reproducible ventricular resection model, we show for the first time that adult newt hearts can fully regenerate without any evidence of scarring. This process is governed by increased proliferation and the up-regulation of cardiac transcription factors normally expressed during developmental cardiogenesis. Furthermore, we are able to identify cells within the newly regenerated regions of the myocardium that express the LIM-homeodomain protein Istet1 and GATA4, transcription factors found in cardiac progenitors. Information acquired from using the newt as a model organism may help to shed light on the regeneration deficits demonstrated in damaged human hearts. (c) 2011 Elsevier Inc. All rights reserved.