The new medium MDSS2N, free of any animal protein supports cell growth and production of various viruses
The new medium MDSS2N, free of any animal protein supports cell growth and production of various viruses
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DOI：
10.1023/a:1008021317639
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发表时间：
1999-01-01
期刊：
影响因子：
2.2
通讯作者：
Perrin, P
中科院分区：
文献类型：
--
作者：
Merten, OW;Kallel, H;Perrin, P
开发不含血清和动物或人蛋白质的培养基对于提高治疗和疫苗接种用生物制剂的安全性至关重要。为了降低污染的风险，我们改良了无血清培养基MDSS 2，这是一种非常有效的无血清培养基，用于生产各种生物制品，包括使用不同细胞系的实验疫苗（Merten等人，1994），通过用植物提取物代替动物衍生产品。新的无血清和动物蛋白培养基（MDSS 2N）可有效地用于各种细胞系的生物量生产。这些细胞在这种新的无血清培养基中的生长与在旧配方（MDSS 2）中的生长一样好或更好：适应MDSS 2N的BHK-21/BRS细胞显示出0.0197 h（-1）（mu（max）= 0.0510 +/- 0.0058 h（-1））的总体比生长率，而在MDSS 2中培养的细胞的平均比生长率为0.0179 h（-1）（mu（max）= 0.0305 +/- 0.0177 h（-1））。Vero细胞在MDSS 2和MDSS 2N中的平均比生长率分别为0.0159 h（-1）和0.0153 h（-1）。MDSS 2和MDSS 2N的比生长速率分别为0.0161 h（-1）和0.0166 h（-1），MDCK细胞在生物反应器中的平均比生长速率MDSS 2N高于MDSS 2; MDSS 2N支持不同病毒生产的能力被同等地评价，并且可以确定对于某些病毒，MDSS 2N和MDSS 2之间没有差异或差异不显著（流感和脊髓灰质炎病毒），而与MDSS 2相比，狂犬病病毒的产生在MDSS 2N中有所减少。使用MDSS 2N细胞培养和各种病毒的生产进行了讨论。
The development of media free of serum and animal or human proteins is of utmost importance for increasing the safety of biologicals produced for therapy and vaccination. In order to reduce the risk of contamination, we have modified the serum free medium MDSS2, a very efficient serum free medium for the production of various biologicals including experimental vaccines using different cell lines (Merten et al., 1994), by replacing the animal derived products by plant extracts. The new serum and animal protein free medium (MDSS2N) can be efficiently used for biomass production of various cell lines. These cells grow equally well or better in this new serum-free medium than in the old formulation (MDSS2):BHK-21/BRS cells, adapted to MDSS2N, showed an overall specific growth rate of 0.0197 h(-1) (mu(max) = 0.0510 +/- 0.0058 h(-1)), whereas those cultivated in MDSS2 grew with an average specific growth rate of 0.0179 h(-1) (mu(max) = 0.0305 +/- 0.0177 h(-1)).Vero cells grew with an average specific growth rate of 0.0159 h(-1) and 0.0153 h(-1) in MDSS2 and MDSS2N, respectively. Very similar growth rates were obtained in microcarrier cultures in stirred tank reactors: the specific growth rates were 0.0161 h(-1) and 0.0166 h(-1) for MDSS2 and MDSS2N cultures, respectively.For MDCK cells, when cultured on microcarriers in bioreactors, a higher average specific growth rate was observed in MDSS2N than in MDSS2; values of 0.0248 h(-1) and 0.0168 h(-1), respectively, were obtained.The capacity of MDSS2N to support the production of different viruses was equally evaluated and it could be established that for certain viruses there are no or insignificant differences between MDSS2N and MDSS2 (influenza and polio virus), whereas, the production of rabies virus is somewhat reduced in MDSS2N when compared to MDSS2. The use of MDSS2N for cell culture and the production of various viruses is discussed.