RNA sequences required for the noncoding function of oskar RNA also mediate regulation of Oskar protein expression by Bicoid Stability Factor.
RNA sequences required for the noncoding function of oskar RNA also mediate regulation of Oskar protein expression by Bicoid Stability Factor.
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DOI：
10.1016/j.ydbio.2015.09.014
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发表时间：
2015-11-15
影响因子：
2.7
通讯作者：
Macdonald PM
中科院分区：
文献类型：
--
作者：
Ryu YH;Macdonald PM
果蝇oskar（osk）mRNA具有编码和非编码功能。作为一种mRNA，osk编码一种蛋白质，该蛋白质特异性地部署在卵母细胞的后部。这种空间受限的部署依赖于mRNA定位程序以及翻译的抑制和激活，所有这些都依赖于主要位于mRNA的3'非翻译区（UTR）中的调控元件。3' UTR还介导osk的非编码功能，这对于卵子发生的进展是必不可少的。最强烈地破坏非编码功能的突变位于mRNA的3'端附近的短区域（C区）中，紧邻激活翻译所需的元件。我们发现，Bicoid稳定因子（BSF）特异性结合到mRNA的C区。bsf的敲除和osk mRNA中BSF结合位点的突变具有相同的结果：Osk表达在卵子发生后期大部分被消除，mRNA定位和翻译都被破坏。尽管osk 3' UTR的C区是非编码功能所必需的，但BSF结合似乎不是该功能所必需的。
The Drosophila oskar (osk) mRNA is unusual in having both coding and noncoding functions. As an mRNA, osk encodes a protein which is deployed specifically at the posterior of the oocyte. This spatially-restricted deployment relies on a program of mRNA localization and both repression and activation of translation, all dependent on regulatory elements located primarily in the 3' untranslated region (UTR) of the mRNA. The 3' UTR also mediates the noncoding function of osk, which is essential for progression through oogenesis. Mutations which most strongly disrupt the noncoding function are positioned in a short region (the C region) near the 3' end of the mRNA, in close proximity to elements required for activation of translation. We show that Bicoid Stability Factor (BSF) binds specifically to the C region of the mRNA. Both knockdown of bsf and mutation of BSF binding sites in osk mRNA have the same consequences: Osk expression is largely eliminated late in oogenesis, with both mRNA localization and translation disrupted. Although the C region of the osk 3' UTR is required for the noncoding function, BSF binding does not appear to be essential for that function.