ALTERATION OF A YEAST SH3 PROTEIN LEADS TO CONDITIONAL VIABILITY WITH DEFECTS IN CYTOSKELETAL AND BUDDING PATTERNS
ALTERATION OF A YEAST SH3 PROTEIN LEADS TO CONDITIONAL VIABILITY WITH DEFECTS IN CYTOSKELETAL AND BUDDING PATTERNS
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DOI：
10.1128/mcb.13.8.5070
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发表时间：
1993-08-01
影响因子：
5.3
通讯作者：
CROUZET, M
中科院分区：
文献类型：
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作者：
BAUER, F;URDACI, M;CROUZET, M
分离出稳定期生存所必需的基因突变，以了解野生型酿酒酵母在长时间营养缺乏期间保持活力的能力。在这里，我们报告的结果，这些突变体之一，rvs 167，这表明降低生存能力和异常的细胞形态后，碳和氮饥饿。当细胞在高盐浓度和其他不利的生长条件下生长时，突变体表现出相同的反应。RVS 167基因产物与Rvs 161蛋白具有显著的同源性，并且在C-末端含有SH 3结构域。异常肌动蛋白分布与突变表型相关。此外，虽然在标准生长条件下单倍体菌株的出芽模式保持轴向，但二倍体突变菌株的出芽模式是随机的。因此，基因RVS 167可能涉及响应于环境胁迫的细胞骨架重组，并可能在出芽位点选择机制中起作用。
Mutations in genes necessary for survival in stationary phase were isolated to understand the ability of wild-type Saccharomyces cerevisiae to remain viable during prolonged periods of nutritional deprivation. Here we report results concerning one of these mutants, rvs167, which shows reduced viability and abnormal cell morphology upon carbon and nitrogen starvation. The mutant exhibits the same response when cells are grown in high salt concentrations and other unfavorable growth conditions. The RVS167 gene product displays significant homology with the Rvs161 protein and contains a SH3 domain at the C-terminal end. Abnormal actin distribution is associated with the mutant phenotype. In addition, while the budding pattern of haploid strains remains axial in standard growth conditions, the budding pattern of diploid mutant strains is random. The gene RVS167 therefore could be implicated in cytoskeletal reorganization in response to environmental stresses and could act in the budding site selection mechanism.