Chrysin induces brown fat-like phenotype and enhances lipid metabolism in 3T3-L1 adipocytes
Chrysin induces brown fat-like phenotype and enhances lipid metabolism in 3T3-L1 adipocytes
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DOI：
10.1016/j.nut.2016.02.007
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发表时间：
2016-09-01
期刊：
影响因子：
4.4
通讯作者：
Yun, Jong Won
中科院分区：
文献类型：
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作者：
Choi, Jae Heon;Yun, Jong Won
目的：许多研究都与有前途的治疗性植物化学物质有关，如类黄酮，以治疗肥胖症和相关并发症，并且已经提出了许多膳食化合物作为增加哺乳动物能量消耗和减少脂肪积累的工具。在这里，我们表明类黄酮白杨素通过增强棕色脂肪特异性基因和蛋白的表达以及增强脂质代谢来诱导3 T3-L1脂肪细胞的布朗宁。方法：通过分别通过实时聚合酶链反应和免疫印迹分析确定棕色脂肪特异性基因和蛋白的表达水平来研究白杨素诱导的脂肪布朗宁。结果：黑曲霉素增强棕色脂肪特异性标志物的表达，并增加过氧化物酶体增殖物激活受体（PPAR）α、PPAR γ、PPAR δ、磷酸化AMP激活蛋白激酶（p-AMPK）、磷酸化乙酰辅酶A羧化酶、激素敏感脂肪酶、围脂蛋白、肉毒碱棕榈酰转移酶1、酰基辅酶A氧化酶1、过氧化物酶体增殖物激活受体-1 α（PGC-1 α）和解偶联蛋白1（UCP-1），表明其可能在增加脂解、脂肪氧化和产热以及减少脂肪生成中起作用。UCP-1和其他棕色脂肪特异性标志物的表达增加可能是由白杨素诱导的AMPK激活介导的，这是基于以下事实：dorsomorphin抑制AMPK消除了含有PR结构域的16、UCP-1和PGC-1 α的表达，而激活剂5-氨基咪唑-4-甲酰胺核糖核苷酸升高了这些棕色标志物蛋白的表达。我们的研究结果表明，白杨素起着双重调节作用的形式，诱导棕色样表型，以及增强脂质代谢，因此可能会被探索作为一个潜在的有前途的食品添加剂，用于预防肥胖。(C)2016 Elsevier Inc. All rights reserved.
Objectives: Many studies have to do with promising therapeutic phytochemicals such as flavonoids to treat obesity and related complications, and a number of dietary compounds have been proposed as tools for increasing energy expenditure and decreasing fat accumulation in mammals. Here, we show that the flavonoid chrysin induces browning of 3T3-L1 adipocytes via enhanced expression of brown fat-specific genes and proteins as well as enhances lipid metabolism.Methods: Chrysin-induced fat browning was investigated by determining expression levels of brown fat-specific genes and proteins by real-time polymerase chain reaction and immunoblot analysis, respectively.Results: Chrysin enhanced expression of brown fat-specific markers and increased protein levels of peroxisome proliferator-activated receptor (PPAR)alpha, PPAR gamma, PPAR delta, phosphorylated AMP-activated protein kinase (p-AMPK), phosphorylated acetyl-CoA carboxylase, hormone sensitive lipase, perilipin, carnitine palmitoyltransferase 1, acyl-coenzyme A oxidase 1, peroxisome proliferator-activated receptor-1 alpha (PGC-1 alpha), and uncoupling protein 1 (UCP-1), suggesting its possible role in augmentation of lipolysis, fat oxidation, and thermogenesis as well as reduction of lipogenesis. Increased expression of UCP-1 and other brown fat-specific markers was possibly mediated by chrysin-induced activation of AMPK based on the fact that inhibition of AMPK by dorsomorphin abolished expression of PR domain-containing 16, UCP-1, and PGC-1 alpha while the activator 5-aminoimidazole-4-carboxamide ribonucleotide elevated expression of these brown marker proteins.Conclusion: Our findings suggest that chrysin plays a dual modulatory role in the form of inducing the brown-like phenotype as well as enhancing lipid metabolism and thus may be explored as a potentially promising food additive for prevention of obesity. (C) 2016 Elsevier Inc. All rights reserved.