Pantoea beijingensis sp nov., isolated from the fruiting body of Pleurotus eryngii
Pantoea beijingensis sp nov., isolated from the fruiting body of Pleurotus eryngii
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DOI：
10.1007/s10482-013-0024-0
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发表时间：
2013-12-01
影响因子：
2.6
通讯作者：
Xu, Feng
中科院分区：
文献类型：
--
作者：
Liu, Yu;Wang, Shouxian;Xu, Feng
从北京软腐病食用菌中国的子实体上分离到4株革兰氏阴性、兼性厌氧细菌。部分16S rRNA基因测序和rpoB部分测序，将这些分离物归入泛胞菌属。基于gyrB、rpoB、InfB和atpD基因部分序列的多位点序列分析表明，弥散泛菌和加维泛亚是它们最近的亲缘关系，表明这些分离物可能是一个新物种。DNA-DNA杂交研究证实，新分离株被归类为一个新物种，表型测试允许与最近的系统发育邻居区分开来。定名为Pantoea beijingensis sp.11月[模式菌株LMG 27579(T)=KCTC 32406(T)=JZB2120001(T)(保存于北京农林科学院植物与环境保护研究所)]。
Four Gram-negative-staining, facultatively anaerobic bacterial isolates were obtained from a fruiting body of the edible mushroom Pleurotus eryngii showing symptoms of soft rot disease in Beijing, China. Partial 16S rRNA gene sequencing, together with partial rpoB sequencing, placed these isolates in the genus Pantoea. Multilocus sequence analysis based on the partial sequences of gyrB, rpoB, infB and atpD revealed Pantoea dispersa and Pantoea gaviniae as their closest phylogenetic relatives and indicated that these isolates constituted a possible novel species. DNA-DNA hybridization studies confirmed the classification of the new isolates as a novel species and phenotypic tests allowed for differentiation from the closest phylogenetic neighbours. The name Pantoea beijingensis sp. nov. [type strain LMG 27579(T) = KCTC 32406(T) = JZB2120001(T) (deposited at Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences)] is proposed.