GLUCOCORTICOID INFLUENCE ON GROWTH OF VASCULAR WALL CELLS IN CULTURE
GLUCOCORTICOID INFLUENCE ON GROWTH OF VASCULAR WALL CELLS IN CULTURE
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DOI：
10.1002/jcp.1041130203
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发表时间：
1982-01-01
影响因子：
5.6
通讯作者：
JOHNSON, LK
中科院分区：
文献类型：
--
作者：
LONGENECKER, JP;KILTY, LA;JOHNSON, LK
研究了牛主动脉内皮细胞和平滑肌细胞的原代大规模培养和克隆株对糖皮质激素的生长反应。原代内皮细胞的生长在不存在成纤维细胞生长因子（FGF）的情况下不受糖皮质激素[ESTA]治疗的影响，但在存在FGF的情况下被抑制约30%;对于克隆的内皮细胞，糖皮质激素也仅在存在FGF的情况下具有生长抑制作用。平滑肌细胞生长抑制30-70%的糖皮质激素治疗在原代培养物和克隆菌株中的FGF的情况下，这种抑制作用被完全废除的两种文化中加入FGF。糖皮质激素对细胞生长的影响是糖皮质激素特异性的，浓度依赖性的，并且观察到与血清浓度无关。显然，糖皮质激素对主动脉平滑肌细胞具有直接和显著的生长抑制作用，但当在体外相同条件下分析血管壁的这些组分时，对内皮细胞的影响很小。
Primary mass cultures and cloned strains of bovine aortic endothelial and smooth muscle cells were investigated with respect to their growth responses to glucocorticoid hormones. The growth of primary endothelial cells was not influenced by glucocorticoid [antiinflammatory] treatment in the absence of fibroblast growth factor (FGF) but was inhibited by about 30% in the presence of FGF; with cloned endothelial cells, glucocorticoids were also growth inhibitory only in the presence of FGF. Smooth muscle cell growth was inhibited 30-70% by glucocorticoid treatment in both primary cultures and in the cloned strains in the absence of FGF, and this inhibition was totally abolished by the addition of FGF for both cultures. The corticosteroid influences on cell growth were glucocorticoid specific, concentration dependent, and were observed to be independent of the serum concentration. Apparently, glucocorticoid hormones have direct and pronounced growth inhibiting effects on aortic smooth muscle cells but only minimal effects on endothelial cells when these components of the vascular wall are analyzed under identical conditions in vitro.