SLXL1, a novel acrosomal protein, interacts with DKKL1 and is involved in fertilization in mice.
SLXL1, a novel acrosomal protein, interacts with DKKL1 and is involved in fertilization in mice.
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SLXL1 是一种新型顶体蛋白，与 DKKL1 相互作用并参与小鼠受精
DOI：
10.1371/journal.pone.0020866
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Han C
中科院分区：
文献类型：
--
作者：
Zhuang XJ;Hou XJ;Liao SY;Wang XX;Cooke HJ;Zhang M;Han C
研究背景精子发生是一个复杂的细胞发育过程，涉及多个基因家族。Xlr（X连锁的淋巴细胞调节的）家族包括多个成员，其中只有少数在减数分裂、减数分裂后成熟和生殖细胞受精中具有功能。Slx-like 1（Slxl 1）是Xlr家族的一员，其在精子发生中的表达和功能尚需进一步研究。方法/主要发现采用RT-PCR、Western blotting和免疫组织化学方法研究了Slxl 1在精子发生不同组织和不同阶段的mRNA和蛋白表达及定位。通过免疫共沉淀和共定位检测SLXL 1的相互作用伴侣。SLXL 1在获能，顶体反应，透明质酸结合/渗透，和受精的作用进行了评估，在体外使用阻断抗血清。结果表明，Slxl 1基因mRNA和蛋白在睾丸中特异表达。SLXL 1仅定位于减数分裂后生殖细胞的顶体中，并与顶体相关蛋白DKKL 1（Dickkopf-like 1）相互作用，DKKL 1在受精过程中起重要作用。抗SLXL 1多克隆抗血清显著降低了透明质酸结合/穿透和受精的速率。结论/意义SLXL 1是XLR家族中第一个与顶体相关的成员，参与精子的透明结合/穿透和随后的受精。这些结果，加上以前的研究，表明Xlr家族成员参与不同的过程中，从减数分裂到受精精子发生。
Background Spermatogenesis is a complex cellular developmental process which involves diverse families of genes. The Xlr (X-linked, lymphocyte regulated) family includes multiple members, only a few of which have reported functions in meiosis, post-meiotic maturation, and fertilization of germ cells. Slx-like1 (Slxl1) is a member of the Xlr family, whose expression and function in spermatogenesis need to be elucidated. Methodology/Principal Findings The mRNA and protein expression and localization of Slxl1 were investigated by RT-PCR, Western blotting and immunohistochemistry in different tissues and at different stages of spermatogenesis. The interacting partner of SLXL1 was examined by co-immunoprecipitation and co-localization. Assessment of the role of SLXL1 in capacitation, acrosome reaction, zona pellucida binding/penetration, and fertilization was carried out in vitro using blocking antisera. The results showed that Slxl1 mRNA and protein were specifically expressed in the testis. SLXL1 was exclusively located in the acrosome of post-meiotic germ cells and interacts with DKKL1 (Dickkopf-like1), which is an acrosome-associated protein and plays an important role in fertilization. The rates of zona pellucida binding/penetration and fertilization were significantly reduced by the anti-SLXL1 polyclonal antiserum. Conclusions/Significance SLXL1 is the first identified member of the XLR family that is associated with acrosome and is involved in zona pellucid binding/penetration and subsequent fertilization. These results, together with previous studies, suggest that Xlr family members participate in diverse processes from meiosis to fertilization during spermatogenesis.
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影响因子：
3.9
作者：
Bendahmane, M;Zeng, HT;Tulsiani, DRP
通讯作者：
Tulsiani, DRP
影响因子：
10.7
作者：
Scavetta, Rick J.;Tautz, Diethard
通讯作者：
Tautz, Diethard
DOI：
10.1073/pnas.1018202108
发表时间：
2011-03-22
影响因子：
11.1
作者：
Jin, Mayuko;Fujiwara, Eiji;Hirohashi, Noritaka
通讯作者：
Hirohashi, Noritaka
影响因子：
64.8
作者：
COHEN, DI;STEINBERG, AD;DAVIS, MM
通讯作者：
DAVIS, MM
影响因子：
5.3
作者：
Pelttari, J;Hoja, MR;Höög, C
通讯作者：
Höög, C