A new leptospiral serovar, ngavi, in the Tarassovi serogroup isolated from Zimbabwe oxen
A new leptospiral serovar, ngavi, in the Tarassovi serogroup isolated from Zimbabwe oxen
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DOI：
10.1099/00207713-48-1-207
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发表时间：
1998-01-01
期刊：
影响因子：
--
通讯作者：
Korver, H
中科院分区：
文献类型：
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作者：
Feresu, SB;Bolin, CA;Korver, H
从津巴布韦屠宰场屠宰的健康牛肾中分离到两株属于Tarassovi血清群的钩端螺旋体属菌株，这两株菌株属于同一血清型，但用交叉凝集素吸收试验不能将其归属于以前已知的血清型。菌株SBF 16是参考菌株，当使用8种单克隆抗体进行分析时，津巴布韦分离物显示出与加图尼血清型的一些抗原相似性，并且当使用RFLP分析分析其染色体DNA时，其限制性图谱与塔拉索维、突尼斯、摩尔达维亚和圭代血清型的相似，用从Hardjo牛血清型中克隆的重复序列元件合成的探针与两个菌株的Southern印迹杂交，可将两个菌株的限制性酶切图谱相互区分开来，并与四个血清型的酶切图谱区分开来。
Two strains of the genus Leptospira, belonging to serogroup Tarassovi, were isolated from kidneys of apparently healthy oxen slaughtered at an abattoir in Zimbabwe, Both strains belonged to the same serovar but could not be assigned to previously known serovars using the cross-agglutinin absorption test, The name ngavi is proposed for the new serovar containing these two strains; strain SBF 16 is the reference strain, The Zimbabwe isolates showed some antigenic similarity to serovar gatuni when analyses were carried out using eight monoclonal antibodies, and had restriction patterns similar to those of serovars tarassovi, tunis, moldaviae and guidae when their chromosomal DNAs were analysed using RFLP analysis, The restriction patterns of the two strains could be distinguished from each other and from those of the four serovars when their Southern blots were hybridized with a probe synthesized from a repetitive sequence element cloned from serovar hardjo strain Hardjo-bovis.