Ovule-specific MADS-box proteins have conserved protein-protein interactions in monocot and dicot plants
Ovule-specific MADS-box proteins have conserved protein-protein interactions in monocot and dicot plants
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DOI：
10.1007/s00438-002-0746-6
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发表时间：
2002-10-01
影响因子：
3.1
通讯作者：
Colombo, L
中科院分区：
文献类型：
--
作者：
Favaro, R;Immink, RGH;Colombo, L
OsMADS13是水稻MADS-box基因，在胚珠发育过程中特异表达。OsMADS13的氨基酸序列与矮牵牛中两个MADS-box基因的产物FBP7和FBP11有74%的相似性，这两个基因是决定矮牵牛胚珠特性的必要条件和充分条件。为了评估水稻FBP7和FBP11的同源基因OsMADS13是否具有相同的功能，进行了几项分析。FBP7和FBP11在矮牵牛中的异位表达导致了在花冠和花瓣上异位胚珠的形成。在这里，我们证明了OsMADS13在水稻和拟南芥中的异位表达并不会导致这种结构的形成。此外，FBP7和FBP11在拟南芥中的异位表达也不能诱导异位胚珠的形成。为了确定与假定的D类MADS-box蛋白有关的蛋白质-蛋白质相互作用是否保守，进行了酵母双杂交试验。这些实验最终确定了OsMADS13的三个假定伙伴，它们都由类似AGL2的基因编码。有趣的是，矮牵牛FBP7蛋白也与AGL2类蛋白相互作用。交换实验证实了这些胚珠特异因子的MADS-box蛋白伙伴的进化保守性，表明OsMADS13的蛋白伙伴与FBP7相互作用，反之亦然。
OsMADS13 is a rice MADS-box gene that is specifically expressed in developing ovules. The amino acid sequence of OsMADS13 shows 74% similarity to those of FLORAL BINDING PROTEIN 7 (FBP7) and FBP11, the products of two MADS-box genes that are necessary and sufficient to determine ovule identity in Petunia. To assess whether OsMADS13, the putative rice ortholog of FBP7 and FBP11, has an equivalent function, several analyses were performed. Ectopic expression of FBP7 and FBP11 in Petunia results in ectopic ovule formation on sepals and petals. Here we show that ectopic expression of OsMADS13 in rice and Arabidopsis does not result in the formation of such structures. Furthermore, ectopic expression of FBP7 and FBP11 in Arabidopsis also fails to induce ectopic ovule formation. To determine whether protein-protein interactions involving putative class D MADS-box proteins have been conserved, yeast two-hybrid assays were performed. These experiments resulted in the identification of three putative partners of OsMADS13, all of them encoded by AGL2-like genes. Interestingly the Petunia FBP7 protein also interacts with AGL2-like proteins. The evolutionary conservation of the MADS-box protein partners of these ovule-specific factors was confirmed by exchange experiments which showed that the protein partners of OsMADS13 interact with FBP7 and vice versa.