Photocleavable Polyethylene Glycol for the Light-Regulation of Protein Function
Photocleavable Polyethylene Glycol for the Light-Regulation of Protein Function
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DOI：
10.1021/bc100084n
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发表时间：
2010-08-01
影响因子：
4.7
通讯作者：
Deiters, Alexander
中科院分区：
文献类型：
--
作者：
Georgianna, Wesleigh E.;Lusic, Hrvoje;Deiters, Alexander
聚乙二醇化通常用于增强蛋白质的药代动力学性质，但它会干扰天然蛋白质功能。因此，蛋白质活性可以通过聚乙二醇化而被消除，并且从蛋白质中除去聚乙二醇（PEG）基团的可控手段是期望的。因此，光通过光敏基团的应用提供了对生物分子的独特控制。本文报道了一种可光裂解的PEG试剂（PhotoPEG）的合成及其在光调控酶活性中的应用。
PEGylation is commonly employed to enhance the pharmacokinetic properties of proteins, but it can interfere with natural protein function. Protein activity can thus be abrogated through PEGylation, and a controllable means to remove the polyethylene glycol (PEG) group from the protein is desirable. As such, light affords a unique control over biomolecules through the application of photosensitive groups. Herein, we report the synthesis of a photocleavable PEG reagent (PhotoPEG) and its application to the light-regulation of enzyme activity.