Behavioral and electrophysiologic responses of Drosophila melanogaster to prolonged periods of anoxia
Behavioral and electrophysiologic responses of Drosophila melanogaster to prolonged periods of anoxia
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DOI：
10.1016/s0022-1910(96)00084-4
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发表时间：
1997-03-01
影响因子：
2.2
通讯作者：
Haddad, GG
中科院分区：
文献类型：
--
作者：
Krishnan, SN;Sun, YA;Haddad, GG
对缺氧的敏感性在不同的门和物种之间差异很大，大多数哺乳动物对低浓度的吸入氧非常敏感，而一些鱼类，海龟和甲壳类动物则非常耐氧。为了确定缺氧耐受性的基础，利用一个模型系统可以产生机械的答案，我们研究了果蝇，果蝇，果蝇被暴露在缺氧环境中5-240分钟，在缺氧1-2分钟后，果蝇失去了协调能力，摔倒，一动不动，然而，它们能忍受完全的氮气环境长达4小时，之后它们就恢复了。缺氧时间和恢复时间之间存在非线性关系，飞行肌对大纤维刺激的细胞外记录显示肌肉诱发反应完全恢复，这种反应在缺氧期间完全不存在，低氧浓度（对照组的20%）下，每克组织的平均O-2消耗大大减少，我们从这些研究中得出结论：(1)黑腹果蝇具有较强的耐氧性，可用于研究其耐氧机制；(2)低氧环境下代谢率的显著下降有助于果蝇的生存。1997爱思唯尔科学有限公司
Sensitivity to anoxia varies tremendously among phyla and species, Most mammals are exquisitely sensitive to low concentrations of inspired oxygen, while some fish, turtles and crustacea are very resistant, To determine the basis of anoxia tolerance, it would be useful to utilize a model system which can yield mechanistic answers, We studied the fruit fly, Drosophila melanogaster, to determine its anoxia resistance since this organism has been previously studied using a variety of approaches and has proven to be very useful in a number of areas of biology, Flies were exposed to anoxia for periods of 5-240 min, and, after 1-2 min in anoxia, Drosophila lost coordination, fell down, and became motionless, However, they tolerated a complete nitrogen atmosphere for up to 4 h following which they recovered, In addition, a nonlinear relation existed between time spent in anoxia and time to recovery, Extracellular recordings from flight muscles in response to giant fiber stimulation revealed complete recovery of muscle-evoked response, a response that was totally absent during anoxia, Mean O-2 consumption per gram of tissue was substantially reduced in low O-2 concentrations (20% of control), We conclude from these studies that: (1) Drosophila melanogaster is very resistant to anoxia and can be useful in the study of mechanisms of anoxia tolerance; and (2) the profound decline in metabolic rate during periods of low environmental O-2 levels contributes to the survival of Drosophila. (C) 1997 Elsevier Science Ltd.