First Report of Citrus exocortis viroid from Grapevine in China.
First Report of Citrus exocortis viroid from Grapevine in China.
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DOI：
10.1094/pdis-94-8-1071a
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发表时间：
2010-07
期刊：
影响因子：
4.5
通讯作者：
J. Shu;G. Wang;W. X. Xu;N. Hong
中科院分区：
文献类型：
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作者：
J. Shu;G. Wang;W. X. Xu;N. Hong
柑橘类外皮病毒（CEVd）可引起柑橘树皮剥落、矮化、叶片膨胀和果实产量损失。柑橘类的CEVd报告来自世界各地，但葡萄类的CEVd报告来自较少的地区（澳大利亚、巴西、加利福尼亚和西班牙）。2009年，研究人员从中国河南、湖北、山东和辽宁省收集了40株不同品种和品种的葡萄叶片。用上述方法(3)从叶片中提取总RNA或双链RNA，用随机引物（Takara, Dalian, China）进行反转录，然后用引物CEV-AM3和CEV-AP3进行PCR(2)。结果表明，仅从两株葡萄的无症状叶片中扩增出372 bp长的目标DNA片段。白玫瑰在湖北省的一个小花园里生长了大约26年。将扩增产物恢复并克隆到pMD18-T （Takara）中，并对每个分离物的10个阳性克隆进行测序和比对。对于这两个分离株，20%的克隆代表相同的变体(CEVd-hn-g-1； GenBank登录号：GU592444)。与NCBI中登记的葡萄变异（GenBank登录号为Y00328.1和DQ471996.1）的最大同源性为94% ~ 99%，与柑橘变异（GenBank登录号为DQ431993.1和DQ831485.1）的最大同源性为91% ~ 100%，与蚕豆变异（GenBank登录号为EF488068.1和EF488050.1）的最大同源性为91% ~ 98%，与番茄变异（GenBank登录号为AY671953.1和S67446.1）的最大同源性为89% ~ 94%。据我们所知，这是中国首例葡萄CEVd报告。参考文献：(1)M. Eiras等。Fitopatol。胸罩。31:40,2006。(2) H. J. Gross等。欧元。生物化学学报，21(1):481 - 481。(3)徐文旭等。j .性研究。方法135:276,2006。
Citrus exocortis viroid (CEVd) can induce bark scaling, dwarfing, leaf epinasty, and fruit yield loss in susceptible hosts. In citrus, CEVd is reported from around the world, but in grape, it is reported from fewer locations (Australia, Brazil, California, and Spain [1]). In 2009, leaves were collected from 40 grapevines (of several different cultivars and species) from Henan, Hubei, Shandong, and Liaoning provinces, China. Total RNA or double-stranded RNA was extracted from the leaves by a described method (3) and subjected to reverse transcription with a random primer (Takara, Dalian, China) and then PCR with primer CEV-AM3 and CEV-AP3 (2). Results showed that the target DNA fragments of 372 bp long were amplified only from the symptomless leaves collected from two grapevines of cv. White Rose grown for approximately 26 years within a small garden in Hubei Province. Amplified products were recovered and cloned into pMD18-T (Takara) and 10 positive clones of each isolate were sequenced and aligned. For both isolates, 20% of the clones represented the same variant (CEVd-hn-g-1; GenBank Accession No. GU592444). It showed a max identity of 94 to 99% with the variants (GenBank Accession Nos. Y00328.1 and DQ471996.1) from grape registered in NCBI, 91 to 100% (GenBank Accession Nos. DQ431993.1 and DQ831485.1) from citrus, 91 to 98% (GenBank Accession Nos. EF488068.1 and EF488050.1) from broad bean, and 89 to 94% (GenBank Accession Nos. AY671953.1 and S67446.1) from tomato. To our knowledge, this is the first report of CEVd from grape in China. References: (1) M. Eiras et al. Fitopatol. Bras. 31:440, 2006. (2) H. J. Gross et al. Eur. J. Biochem. 121:249, 1982. (3) W. X. Xu et al. J. Virol. Methods 135:276, 2006.