Prevalence of Salmonella enterica serovars and genovars from chicken carcasses in slaughterhouses in Spain
Prevalence of Salmonella enterica serovars and genovars from chicken carcasses in slaughterhouses in Spain
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DOI：
10.1111/j.1365-2672.2007.03368.x
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发表时间：
2007-11-01
影响因子：
4
通讯作者：
Prieto, M.
中科院分区：
文献类型：
--
作者：
Capita, R.;Alonso-Calleja, C.;Prieto, M.
目的：确定西班牙屠宰场鸡尸体中肠道沙门氏菌血清型的流行率，并检查这些血清型之间的基因型关系。方法和结果：从西班牙西北部的六个屠宰场共收集了336只鸡尸体。分离沙门氏菌（ISO- 6579- 1993），针对20种抗生素进行血清分型、噬菌体分型、核糖分型和抗微生物分型。沙门氏菌菌株检出60（17中心点9%）尸体。分离株属于9个不同的血清型，Salm。肠道感染最常见。3株（5%）对一种抗生素耐药，24株（40%）对多种抗生素耐药。最常见的耐药是磺胺类、氟喹诺酮类和四环素类。核糖分型能够区分相同血清型和噬菌体类型的分离株。结论：沙门氏菌血清型和噬菌体类型检测是最常见的与人类疾病在西班牙。抗生素耐药菌株的比例很大，这是一个令人关切的问题。来自不同屠宰场的菌株之间的高度遗传关系被发现。意义和影响的研究：这项研究提供了详细的信息沙门氏菌在西班牙的家禽分离株。它强调了在家禽产品中控制这种病原体的重要性，并建议需要更谨慎地使用抗生素。
Aims: To determine the prevalence of Salmonella enterica serovars in chicken carcasses in slaughterhouses in Spain and to examine genotypic relations among these serovars. Methods and Results: A total of 336 chicken carcasses were collected from six slaughterhouses in Northwestern Spain. Salmonellae were isolated ( ISO- 6579- 1993), serotyped, phage- typed, ribotyped and antibiotyped against 20 antibiotics. Salmonella strains were detected in 60 ( 17 center dot 9%) carcasses. Isolates belonged to nine different serotypes, with Salm. Enteritidis being the most common. Three strains ( 5%) were resistant to one antibiotic and 24 ( 40%) were multi- resistant ( to more than one antibiotic). The most frequently encountered resistances were to sulphamides, fluoroquinolones and tetracycline. Ribotyping was able to differentiate isolates of the same serotype and phage type. Conclusions: The Salmonella serotypes and phage types detected are among those most frequently associated with human diseases in Spain. The large percentage of antimicrobial resistant strains is a matter for concern. A high genetic relationship between strains from different slaughterhouses was found. Significance and Impact of the Study: This study provides detailed information about Salmonella isolates from poultry in Spain. It emphasizes the importance of controlling this pathogen in poultry products, and suggests the need for more prudent use of antibiotics.