Defining inoculation conditions for the mouse model of ascending urinary tract infection that avoid immediate vesicoureteral reflux yet produce renal and bladder infection
Defining inoculation conditions for the mouse model of ascending urinary tract infection that avoid immediate vesicoureteral reflux yet produce renal and bladder infection
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DOI：
10.1093/infdis/173.3.746
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发表时间：
1996-03-01
影响因子：
6.4
通讯作者：
Brown, JJ
中科院分区：
文献类型：
--
作者：
Johnson, JR;Brown, JJ
令人满意的上行尿路感染（UTI）小鼠模型必须避免接种引起的膀胱输尿管反流（VUR），但仍然在相当大比例的小鼠中产生肾脏和膀胱感染，为了定义满足这两个条件的接种条件，在各种条件下接种后立即评估小鼠的VUR，并在接种后48小时评估肾脏和膀胱感染，消除VUR需要减慢输注速度，减少接种量（25 mu L），并使用创伤性较小的方法进行安乐死和器官收获，在无 VUR 条件下接种 2 种野生型大肠杆菌菌株中的任何一种的小鼠中，膀胱和肾脏感染在 48 小时内非常普遍。与其他研究人员的报告一起，这些发现表明，上行性 UTI 小鼠模型的令人满意的实验条件可以根据经验确定，但可能是实验室特定的。
A satisfactory mouse model of ascending urinary tract infection (UTI) must avoid inoculation-induced vesicoureteral reflux (VUR) yet still produce kidney and bladder infection in a substantial proportion of mice, To define inoculation conditions that would satisfy both these conditions, mice were evaluated for VUR immediately after inoculation under a variety of conditions and were assessed for kidney and bladder infection 48 h after inoculation, Elimination of VUR required a slowed infusion rate, a reduced inoculum volume (25 mu L), and use of less traumatic methods for euthanasia and organ harvest, Bladder and kidney infection were highly prevalent at 48 h among mice inoculated under VUR-free conditions with either of 2 wild type Escherichia coli strains, Together with reports from other investigators, these findings indicate that satisfactory experimental conditions for the mouse model of ascending UTI can be defined empirically but may be laboratory-specific.