Reovirus preferentially infects the basolateral surface and is released from the apical surface of polarized human respiratory epithelial cells
Reovirus preferentially infects the basolateral surface and is released from the apical surface of polarized human respiratory epithelial cells
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DOI：
10.1086/529515
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发表时间：
2008-04-15
影响因子：
6.4
通讯作者：
Zabner, Joseph
中科院分区：
文献类型：
--
作者：
Excoffon, Katherine J. D. A.;Guglielmi, Kristen M.;Zabner, Joseph
哺乳动物呼肠孤病毒感染呼吸道和胃肠道上皮细胞，并导致新生儿患病。交界性黏附分子-A(JAM-A)是呼肠孤病毒的非血清型受体。JAM-A定位于紧密连接，并有助于极化上皮的细胞旁通透性。为了研究呼肠孤病毒感染极化上皮细胞的机制，我们评估了呼肠孤病毒在原代培养的人呼吸道上皮细胞顶端和基底侧吸附后的复制、释放和传播。呼肠孤病毒感染人呼吸道上皮细胞，吸附到基底面后比吸附到根尖面后更有效，并依赖于JAM-A。呼肠孤病毒与碳水化合物受体唾液酸结合可抑制根尖感染，神经氨酸酶处理可部分改善这种作用。尽管倾向于基底外侧感染，呼肠孤病毒从呼吸道上皮细胞的顶端表面释放出来，并没有破坏紧密连接。这些结果证实了呼肠孤病毒在极化的人呼吸道上皮细胞中存在感染电路。
Mammalian reoviruses infect respiratory and gastrointestinal epithelia and cause disease in neonates. Junctional adhesion molecule-A (JAM-A) is a serotype-independent receptor for reovirus. JAM-A localizes to tight junctions and contributes to paracellular permeability in polarized epithelia. To investigate the mechanisms of reovirus infection of polarized epithelial cells, we assessed reovirus replication, release, and spread after apical and basolateral adsorption to primary human airway epithelial cultures. Reovirus infection of human airway epithelia was more efficient after adsorption to the basolateral surface than after adsorption to the apical surface, and it was dependent on JAM-A. Reovirus binding to carbohydrate coreceptor sialic acid inhibited apical infection, which was partially ameliorated by treatment of the cultures with neuraminidase. Despite the preference for basolateral infection, reovirus was released from the apical surface of respiratory epithelia and did not disrupt tight junctions. These results establish the existence of an infectious circuit for reovirus in polarized human respiratory epithelial cells.