Fine-structure genetic map of the maltose transport operon of Salmonella typhimurium.
Fine-structure genetic map of the maltose transport operon of Salmonella typhimurium.
复制标题
鼠伤寒沙门氏菌麦芽糖转运操纵子的精细结构遗传图谱。
DOI：
10.1128/jb.171.11.5860-5865.1989
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发表时间：
1989
影响因子：
3.2
通讯作者：
Ames,GF
中科院分区：
文献类型：
--
作者：
Schneider,E;Bishop,L;Schneider,E;Alfandary,V;Ames,GF
我们构建了鼠伤寒沙门氏菌麦芽糖转运操纵子的精细结构遗传图谱。我们已经通过指示板、青霉素选择或质子自杀技术分离出了突变株。突变体可以自发获得，也可以通过化学诱变和转座子插入获得。Tn10和Mu d(Lac AP)1插入突变用于产生缺失。在编码Lambda噬菌体受体的大肠杆菌中，也获得了相当于Lamb的基因突变。用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法和免疫印迹法对突变体的基因产物进行了鉴定。我们的数据表明，该操纵子在沙门氏菌染色体上的位置以及基因顺序和方向与大肠杆菌中的相同。该图谱有助于研究鼠伤寒沙门氏菌的周质转运机制。
We have constructed a fine-structure genetic map of the maltose transport operon in Salmonella typhimurium. We have isolated mal mutants by using indicator plates, penicillin selection, or a proton suicide technique. Mutants were obtained as spontaneous events or were induced by chemical mutagenesis and transposon insertion. Tn10 and Mu d(lac Ap)1 insertion mutations were used to create deletions. Mutations were also obtained in a gene that is equivalent to lamB in Escherichia coli, which codes for the lambda bacteriophage receptor. The gene products in the mutants were characterized by sodium dodecyl sulfate-polyacrylamide-gel electrophoresis and immunoblotting. Our data indicate that the location of this operon on the Salmonella chromosome as well as the gene order and its orientation are the same as those in E. coli. This map will be useful in studying the mechanism of periplasmic transport in S. typhimurium.