Functionalization of whole-cell bacterial reporters with magnetic nanoparticle.
Functionalization of whole-cell bacterial reporters with magnetic nanoparticle.
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DOI：
10.1111/j.1751-7915.2010.00228.x
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发表时间：
2011-01
影响因子：
5.7
通讯作者：
Huang WE
中科院分区：
文献类型：
--
作者：
Zhang D;Fakhrullin RF;Özmen M;Wang H;Wang J;Paunov VN;Li G;Huang WE
我们开发了一种生物相容性和高效的方法，用于利用磁性纳米颗粒（MNP）功能化细菌细胞壁。三种基于Baylyi不动杆菌ADP 1染色体的生物报告基因经过基因工程改造，能够响应水杨酸盐、甲苯/二甲苯和烷烃而表达生物发光，用18 ± 3 nm的氧化铁MNP功能化以获得磁性功能。 MNP功能化不动杆菌生物报告基因的效率为99.96 ± 0.01%。 MNP功能化的生物报告分子（MFB）可以通过外部磁场进行远程控制和收集。在灵敏度、特异性和定量反应方面，MFB都是活的，并且功能与天然细胞一样好。更重要的是，我们证明了水杨酸盐传感MFB可以应用于沉积物和花园土壤，并通过用永磁体可分辨地回收MFB来半定量检测这些样品中的水杨酸盐。磁功能化细胞特别适用于复杂环境，其中固有细胞、颗粒和杂质可能干扰生物报告细胞的直接测量，并且常规过滤不适用于区分和收获生物报告细胞。这里描述的方法提供了一个强大的工具来远程控制和选择性地操纵水和土壤中的MNP功能化细胞。它在环境微生物学的应用中具有很大的潜力，如生物修复强化和环境监测与评价。
We developed a biocompatible and highly efficient approach for functionalization of bacterial cell wall with magnetic nanoparticles (MNPs). Three Acinetobacter baylyi ADP1 chromosomally based bioreporters, which were genetically engineered to express bioluminescence in response to salicylate, toluene/xylene and alkanes, were functionalized with 18 ± 3 nm iron oxide MNPs to acquire magnetic function. The efficiency of MNPs functionalization of Acinetobacter bioreporters was 99.96 ± 0.01%. The MNPs‐functionalized bioreporters (MFBs) can be remotely controlled and collected by an external magnetic field. The MFBs were all viable and functional as good as the native cells in terms of sensitivity, specificity and quantitative response. More importantly, we demonstrated that salicylate sensing MFBs can be applied to sediments and garden soils, and semi‐quantitatively detect salicylate in those samples by discriminably recovering MFBs with a permanent magnet. The magnetically functionalized cells are especially useful to complex environments in which the indigenous cells, particles and impurities may interfere with direct measurement of bioreporter cells and conventional filtration is not applicable to distinguish and harvest bioreporters. The approach described here provides a powerful tool to remotely control and selectively manipulate MNPs‐functionalized cells in water and soils. It would have a potential in the application of environmental microbiology, such as bioremediation enhancement and environment monitoring and assessment.
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影响因子：
4.9
作者：
Kuo, Wen-Shuo;Wu, Ching-Ming;Yeh, Chen-Sheng
通讯作者：
Yeh, Chen-Sheng
影响因子：
5.1
作者：
Huang, WE;Wang, H;Whiteley, AS
通讯作者：
Whiteley, AS
影响因子：
--
作者：
Dyab, Amro K. F.;Ozmen, Mustafa;Paunov, Vesselin N.
通讯作者：
Paunov, Vesselin N.
影响因子：
11.4
作者：
Song, Yizhi;Li, Guanghe;Huang, Wei E.
通讯作者：
Huang, Wei E.
影响因子：
64.8
作者：
Ro, DK;Paradise, EM;Keasling, JD
通讯作者：
Keasling, JD