Finite Element Modeling Predictions of Region-specific Cell-matrix Mechanics in the Meniscus
Finite Element Modeling Predictions of Region-specific Cell-matrix Mechanics in the Meniscus
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DOI：
10.1007/s10237-006-0031-4
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发表时间：
2006-03
影响因子：
3.5
通讯作者：
M. L. Upton;F. Guilak;T. Laursen;L. Setton
中科院分区：
文献类型：
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作者：
M. L. Upton;F. Guilak;T. Laursen;L. Setton
膝关节半月板的生化成分和细胞形态表现出显着的空间变化，反映了位于径向内部和外部区域的细胞的不同表型。与这些细胞表型相关的是空间异质的微观结构和机械环境，最内部区域比外部区域经历更高的流体压力和更低的拉伸应变。然而，目前尚不清楚半月板组织力学如何与细胞的局部微机械环境相关。在这项研究中，理论模型的发展，以研究力学的内部和外部弯月形细胞与不同的几何形状。施加双轴应变的结果预测细胞力学环境中的显著区域差异，圆形内部细胞沿胶原纤维方向的拉伸应变沿着为~0.07，而细长外部半月板细胞的拉伸应变水平为0.02-0.04。结果表明，细胞外基质各向异性和细胞形态在调节半月板细胞的区域特异性微观力学中具有重要的机械作用，这可能进一步在调节细胞对机械刺激的反应中发挥作用。
The knee meniscus exhibits significant spatial variations in biochemical composition and cell morphology that reflect distinct phenotypes of cells located in the radial inner and outer regions. Associated with these cell phenotypes is a spatially heterogeneous microstructure and mechanical environment with the innermost regions experiencing higher fluid pressures and lower tensile strains than the outer regions. It is presently unknown, however, how meniscus tissue mechanics correlate with the local micromechanical environment of cells. In this study, theoretical models were developed to study mechanics of inner and outer meniscus cells with varying geometries. The results for an applied biaxial strain predict significant regional differences in the cellular mechanical environment with evidence of tensile strains along the collagen fiber direction of ~0.07 for the rounded inner cells, as compared to levels of 0.02–0.04 for the elongated outer meniscus cells. The results demonstrate an important mechanical role of extracellular matrix anisotropy and cell morphology in regulating the region-specific micromechanics of meniscus cells, that may further play a role in modulating cellular responses to mechanical stimuli.