Highly reproducible method of planar lipid bilayer reconstitution in polymethyl methacrylate microfluidic chip
Highly reproducible method of planar lipid bilayer reconstitution in polymethyl methacrylate microfluidic chip
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DOI：
10.1021/la052534p
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发表时间：
2006-02-14
期刊：
影响因子：
3.9
通讯作者：
Takeuchi, S
中科院分区：
文献类型：
--
作者：
Suzuki, H;Tabata, KV;Takeuchi, S
我们开发了一种高重复性的平面脂质双层重构方法，使用由聚甲基丙烯酸甲酯（PMMA）塑料基板制成的微流控系统。通过将脂质溶液和缓冲液交替流入集成微流体通道，在直径为100 μ m的孔处形成平面脂质双层。由于孔径处脂质溶液的数量和分布决定了脂质双分子层的状态，因此精确控制它们是至关重要的。我们设计了流体系统的几何形状，使油脂溶液在孔径处分布恒定。然后外压使脂质溶液层变薄，在200-400 Pa的压力下最终成为双分子层。地层过程可以通过光学和电子记录同时监测。双分子层形成的最大收率为90%。使用这种技术，四个脂质双分子层在一个芯片中同时形成。最后，记录了通过革兰西菌肽离子通道的通道电流，证明了芯片与单分子电生理的兼容性。
We developed a highly reproducible method for planar lipid bilayer reconstitution using a microfluidic system made of a polymethyl methacrylate (PMMA) plastic substrate. Planar lipid bilayers are formed at apertures, 100 mu m in diameter, by flowing lipid solution and buffer alternately into an integrated microfluidic channel. Since the amount and distribution of the lipid Solution at the aperture determines the state of the lipid bilayer, controlling them precisely is crucial. We designed the geometry of the fluidic system so that a constant amount of lipid solution is distributed at the aperture. Then, the layer of lipid solution was thinned by applying an external pressure and finally became a bilayer when a pressure of 200-400 Pa was applied. The formation process can be simultaneously monitored with optical and electrical recordings. The maximum yield for bilayer formation was 90%. Using this technique, four lipid bilayers are formed simultaneously in a single chip. Finally, a channel Current through gramicidin peptide ion channels was recorded to prove the compatibility of the chip with single molecule electrophysiology.