HLA-G, -E, -F preworkshop: Tools and protocols for analysis of non-classical class I genes transcription and protein expression
HLA-G, -E, -F preworkshop: Tools and protocols for analysis of non-classical class I genes transcription and protein expression
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DOI：
10.1016/s0198-8859(00)00154-3
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发表时间：
2000-11-01
期刊：
影响因子：
2.7
通讯作者：
Carosella, ED
中科院分区：
文献类型：
--
作者：
Paul, P;Rouas-Freiss, N;Carosella, ED
非经典 MHC I 类 HLA-E、-F 和 -G 分子与经典 I 类组织相容性抗原的不同之处在于特定的转录模式、蛋白质表达和免疫功能。这些非经典抗原表达模式的限制可能在怀孕和疾病期间免疫反应的调节中发挥关键作用，但仍有待进一步定义。第二届HLA-G国际会议和第13届HLA-G组织相容性研讨会的一个具体组成部分将致力于分析正常和病理组织中非经典I类基因的转录和表达。第一步，称为预研讨会，我们在这里报告一个工作组的分析和结论，该工作组的成立是为了收集和验证最佳试剂和方案，允许对 HLA-E、-F、-G 转录水平进行 RT-PCR 分析，并对细胞和组织中的 HLA-G 表达进行流式细胞术和免疫化学分析。这项工作的结果是，使用特异性引物和探针检测 HLA-E、-F 和 G 的替代转录物已在表达 HLA I 类抗原差异模式的转染细胞中得到验证。对收集的抗体的特异性和亲和力的分析允许在研讨会期间提出用于正常和病理组织中 HLA-G 表达的免疫化学和流式细胞术分析的试剂的定义。这项工作允许组建一个扩展的研讨会小组，该小组现在正在开始分析各种细胞和组织中的非经典 I 类转录和表达，这是一项集体贡献，将进一步完善我们对这些抗原在正常和病理情况下表达的看法。 (C) 美国组织相容性和免疫遗传学学会，2000 年。由 Elsevier Science Inc. 出版。
Non-classical MHC class I HLA-E, -F, and -G molecules differ from classical class I histocompatibility antigens by specific patterns of transcription, protein expression, and immunological functions. Restriction of the expression pattern of these non-classical antigens may play a key role in modulation of immune responses during pregnancy and diseases but remains to be additionally defined. A specific component of the second International Conference on HLA-G and the 13th HLA-G Histocompatibility Workshop will be dedicated to the analysis of transcription and expression of non-classical class I genes in normal and pathological tissues. In a first step, referred to as the preworkshop, we here report the analysis and conclusions of a working group which was constituted to gather and validate optimal reagents and protocols allowing RT-PCR analysis of HLA-E, -F, -G transcript levels and flow cytometry and immunochemistry analysis of HLA-G expression in cells and tissues. As a result of this work, use of specific primers and probes detecting alternative transcripts of HLA-E, -F, and G have been validated in transfected cells expressing differential pattern of HLA class I antigens, Analysis of the specificity and affinity of collected antibodies has allowed definition of reagents to be proposed for immunochemistry and flow cytometry analysis of HLA-G expression in normal and pathological tissues during the workshop. This work has allowed constitution of an extended workshop group which is now initiating analysis of non-classical class I transcription and expression in various cells and tissues, a collective contribution that will additionally refine our view of the expression of these antigens in normal and pathological situations. (C) American Society for Histocompatibility and Immunogenetics, 2000. Published by Elsevier Science Inc.