Electrolytes in the axoplasm of the giant nerve fibers of the squid
Electrolytes in the axoplasm of the giant nerve fibers of the squid
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DOI：
10.1002/jcp.1030140208
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发表时间：
1939-12-01
影响因子：
--
通讯作者：
Schmitt, FO
中科院分区：
文献类型：
--
作者：
Bear, RS;Schmitt, FO
通过使用鱿鱼巨大的神经纤维，通过从戏弄和结扎的单个巨大纤维的切割末端挤压，可以获得几乎没有被细胞外物质污染的轴质。对这种分离的轴质的分析表明，K存在于圆锥中。血液中的含量是这个的18倍。总而言之。无机离子只占血液的63%，阳离子的浓度是阴离子（主要是氯离子）的4到5倍。这种阴离子的缺乏（约0.5摩尔）可能是由中等离子质量的有机阴离子弥补的。在轴质中，虽然含量低，但仍是可观的，每克轴质水的含量为0.13毫克，约为血液的25%。通过使用鱿鱼巨大的神经纤维，通过从戏弄和结扎的单个巨大纤维的切割末端挤压，可以获得几乎没有被细胞外物质污染的轴质。对这种分离的轴质的分析表明，K存在于圆锥中。血液中的含量是这个的18倍。总而言之。无机离子只占血液的63%，阳离子的浓度是阴离子（主要是氯离子）的4到5倍。这种阴离子的缺乏（约0.5摩尔）可能是由中等离子质量的有机阴离子弥补的。在轴质中，虽然含量低，但仍是可观的，每克轴质水的含量为0.13毫克，约为血液的25%。
By the use of squid giant nerve fibers it was possible, by extrusion from the cut ends of teased and ligated single giant fibers, to obtain axoplasm virtually uncontaminated with extracellular material. Analysis of such isolated axoplasm shows that K is present in a conc. about 18 times that in the blood. The total conc. of inorganic ions is only 63% that of the blood, cations being 4 to 5 times as concentrated as anions (chiefly chloride). This deficiency of anions (about 0.5 molal) is probably made up by organic anions of moderate ionic wt. The chloride conc. in axoplasm, while low, is appreciable, being 0.13 m. eq. per g. of axoplasm water, or about 25% that of the blood.By the use of squid giant nerve fibers it was possible, by extrusion from the cut ends of teased and ligated single giant fibers, to obtain axoplasm virtually uncontaminated with extracellular material. Analysis of such isolated axoplasm shows that K is present in a conc. about 18 times that in the blood. The total conc. of inorganic ions is only 63% that of the blood, cations being 4 to 5 times as concentrated as anions (chiefly chloride). This deficiency of anions (about 0.5 molal) is probably made up by organic anions of moderate ionic wt. The chloride conc. in axoplasm, while low, is appreciable, being 0.13 m. eq. per g. of axoplasm water, or about 25% that of the blood.