Improvement of Ganoderic Acid and Ganoderma Polysaccharide Biosynthesis by Ganoderma lucidum Fermentation Under the Inducement of Cu2+
Improvement of Ganoderic Acid and Ganoderma Polysaccharide Biosynthesis by Ganoderma lucidum Fermentation Under the Inducement of Cu2+
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Cu2+诱导灵芝发酵提高灵芝酸和灵芝多糖生物合成
DOI：
10.1002/btpr.352
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发表时间：
2010-03-01
影响因子：
2.9
通讯作者：
Zhu, Li-Wen
中科院分区：
文献类型：
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作者：
Tang, Ya-Jie;Zhu, Li-Wen
在药用真菌灵芝深层发酵过程中添加Cu 2+可显著促进灵芝细胞的生长和生物活性物质的积累。采用响应面法结合全因子设计建立数学模型，研究了Cu 2+添加浓度和添加时间的协同效应。Cu 2+对灵芝细胞生长的最佳诱导策略与对灵芝酸（GA）和灵芝多糖生物合成的最佳诱导策略不同。开发了通过在第2、6、8天添加各ImM Cu 2+和在第4天添加2 mM Cu 2+的Cu 2+的多次添加策略以增强GA和胞外多糖的总积累。赤霉素含量最高可达3.0 ± 0.1mg/100 mg干重，比未加Cu ~（2+）的对照提高了76.5%，比所建数学模型预测的峰值提高了33.9%。而相对较高的Cu 2+添加浓度（即，5 mM）对培养第4天的细胞内多糖的含量和总产量均较高。© 2009年美国化学工程师学会生物技术。程序，2010
The cell growth and total accumulation of bioactive metabolites were significantly improved by Cu2+ addition during the submerged fermentation of medicinal mushroom Ganoderma lucidum. A mathematical model, constructed by response surface methodology combination with full factorial design, was applied to study the synergic effect of Cu2+ addition concentration and addition time. The optimal Cu2+ inducement strategy for the cell growth were different from those for the biosynthesis of ganoderic acid (GA) and Ganoderma polysaccharide. A multiple additions strategy of Cu2+ by adding each 1 mM Cu2+ on day 2, 6, 8 and 2 mM Cu2+ on day 4 was developed to enhance total accumulation of GA and extracellular polysaccharides. The highest GA content reached 3.0 ± 0.1 mg per 100 mg DW, which was increased by 76.5% and 33.9% compared with the control without Cu2+ addition and the peak value predicted by the constructed mathematical model, respectively. While, relatively higher addition concentration of Cu2+ (i.e., 5 mM) on the culture of day 4 led to higher content and total production of intracellular polysaccharides. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2010