Aptamers with fluorescence-signaling properties
Aptamers with fluorescence-signaling properties
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DOI：
10.1016/j.ymeth.2005.07.001
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发表时间：
2005-09-01
期刊：
影响因子：
4.8
通讯作者：
Li, YF
中科院分区：
文献类型：
--
作者：
Nutiu, R;Li, YF
适配子是具有配体结合能力的单链DNA或RNA分子。信号适配子是指具有可记录信号产生能力的适配子或修饰适配子。特别是，荧光信号适配子是有价值的分子工具，可用于建立重要的技术或分析，以询问蛋白质和代谢物的身份和浓度。由于标准DNA和RNA适配子本身并不是固有的荧光，为了合理地将非荧光适配子转化为荧光报告分子或通过体外选择直接从随机序列DNA文库中选择荧光适配子，需要采用修饰方法。本文将简要回顾各种信号适体设计策略，并详细描述可用于通过合理设计和体外选择来产生一类特殊类型的信号适体的方法，称为结构切换信号适体。这类信号适配子被设计成通过将结构从预先形成的低荧光双链组装切换到具有更高荧光水平的配体-适配子复合体来发挥作用。(C)2005 Elsevier Inc.保留所有权利。
Aptamers are single-stranded DNA or RNA molecules with ligand-binding capabilities. Signaling aptamers refer to aptamers or modified aptamers with recordable signal generation ability. Fluorescence-signaling aptamers, in particular, are valuable molecular tools that can be used to establish important techniques or assays for the interrogation of identities and concentrations of proteins and metabolites. Since standard DNA and RNA aptamers themselves are not inherently fluorescent, modification methods are required for rationally converting non-fluorescent aptamers into fluorescent reporters or for selecting fluorescent aptamers directly from random-sequence DNA libraries by in vitro selection. This article will provide a brief review of various signaling aptamer design strategies as well as a detailed description of methods that can be used to generate, by both rational design and in vitro selection, a special class of signaling aptamers dubbed "structure-switching signaling aptamers." This class of signaling aptamers are designed to function by switching structures from a pre-formed, lowly fluorescent duplex assembly to a ligand-aptamer complex having a higher level of fluorescence. (c) 2005 Elsevier Inc. All rights reserved.