IDENTIFICATION OF THE NEURITOGEN FOR EXPERIMENTAL ALLERGIC NEURITIS
IDENTIFICATION OF THE NEURITOGEN FOR EXPERIMENTAL ALLERGIC NEURITIS
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DOI：
10.1038/277140a0
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发表时间：
1979-01-01
期刊：
影响因子：
64.8
通讯作者：
HUGHES, RAC
中科院分区：
文献类型：
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作者：
KADLUBOWSKI, M;HUGHES, RAC
实验性变应性神经炎（EAN）是人类急性炎症性多发性神经病（格林-巴利综合征）的模型。它可以在几种实验室动物中通过用在弗氏完全佐剂中乳化的同源或异源外周神经免疫来产生1，2。大约两周后，动物可能出现从共济失调到瘫痪和死亡的各种体征。EAN已经使用外周神经的粗酸提取物产生3，4，但是对外周神经髓鞘的组分蛋白的神经突发生特性的研究已经产生了不确定的结果5 -10。在实验性变态反应性脑脊髓炎（EAE）中，EAN的中枢神经系统（CNS）对应物，致脑炎抗原已被证明是分子量为18，000的CNS髓鞘碱性蛋白（参考文献11）。周围神经髓鞘的三种主要蛋白质，它们共占髓鞘蛋白质的约75%，通常被称为P0、P1和P2（参考文献12）。P0是一种分子量为30，000的糖蛋白，P1和P2都是碱性蛋白，分子量分别为18，000和12，000 - 14，000 12 -13。我们在这里描述了一个可靠的方法，生产严重的EAN在近交系刘易斯大鼠与牛硬膜内根材料，并表明，完整的P2，周围神经髓鞘的主要碱性蛋白，是负责neuritogen。
EXPERIMENTAL ALLERGIC NEURITIS (EAN) is a model of human acute inflammatory polyneuropathy, Guillain–Barré syndrome. It can be produced in several species of laboratory animal by immunisation with homologous or heterologous peripheral nerve emulsified in Freund's complete adjuvant1,2. After about two weeks the animals may develop signs varying from ataxia to paralysis and death. EAN has been produced using crude acid extracts of peripheral nerve3,4, but investigations into the neuritogenic properties of the component proteins of peripheral nerve myelin have yielded inconclusive results5–10. In experimental allergic encephalomyelitis (EAE), the central nervous system (CNS) counterpart of EAN, the encephalitogenic antigen has been shown to be a CNS myelin basic protein of molecular weight 18,000 (ref. 11). The three major proteins of peripheral nerve myelin, which together account for about 75% of the myelin protein, are usually referred to as P0, P1and P2(ref. 12). P0is a glycoprotein of molecular weight 30,000 and P1and P2are both basic proteins with molecular weights of 18,000 and 12,000–14,000, respectively12–13. We describe here a reliable method for producing severe EAN in inbred Lewis rats with bovine intradural root material, and show that intact P2, the major basic protein of peripheral nerve myelin, is the responsible neuritogen.