RNA splicing in neurospora mitochondria: Structure of the unspliced 35S precursor ribosomal RNA detected by psoralen cross-linking
RNA splicing in neurospora mitochondria: Structure of the unspliced 35S precursor ribosomal RNA detected by psoralen cross-linking
复制标题
神经孢子虫线粒体中的 RNA 剪接：通过补骨脂素交联检测到的未剪接 35S 前体核糖体 RNA 的结构
DOI：
10.1016/0092-8674(83)90459-2
复制
发表时间：
1983
期刊：
影响因子：
64.5
通讯作者：
A. Lambowitz
中科院分区：
文献类型：
--
作者：
P. Wollenzien;C. Cantor;D. Grant;A. Lambowitz
用光化学交联法研究了脉孢菌线粒体未剪接的3%前体rRNA的结构。结果表明，当35 s RNA在适当条件下交联在核糖核蛋白颗粒（RNP）中时，主要构型是2.2kb的内含子环，其使相反的剪接位点靠近;游离RNA的主要二级结构特征是相对大的发夹（长度= 0.105 kb）;内含子环和中心发夹是5 '剪接位点处或附近的序列的不同构型;并且内含子环由RNP的蛋白质组分稳定。基于检测到的结构pepsilen光化学交联，我们提出了一种机制的链孢霉线粒体前体rRNA的剪接。我们进一步提出，这种机制的某些功能可能与其他RNA的剪接有关，包括真核mRNA。
The structure of the unspliced 3% precursor rRNA of Neurospora mitochondria was studied by psoralen photochemical cross-linking. The results show that when the 35s RNA is cross-linked in ribonucleoprotein particles (RNPs) under appropriate conditions, the predominant configuration is a 2.2 kb intron loop which brings opposite splice sites into proximity; that the predominant secondary structural feature in the free RNA is a relatively large hairpin (length= 0.105 kb) in the center of the molecule at or near the 5’splice site; that the intron loop and the central hairpin are different configurations of sequences at or near the 5’splice site; and that the intron loop is stabilized by protein components of RNPs. Based on the structures detected by psoralen photochemical cross-linking, we propose a mechanism for the splicing of the Neurospora mitochondrial precursor rRNA. We propose further that certain features of this mechanism may be relevant to the splicing of other RNAs, including eucaryotic mRNAs.
脉孢菌线粒体中的核糖核酸剪接：通过核糖核酸酶 III 消化和电子显微镜研究 35S 核糖体前体核糖核酸的二级结构。
影响因子：
2.9
作者：
Grimm,MF;Cole,MD;Lambowitz,AM
通讯作者：
Lambowitz,AM
粗糙脉孢菌线粒体 DNA 的核糖体 RNA 和转移 RNA 区域的转录本和处理模式。
DOI：
--
发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Green,MR;Grimm,MF;Goewert,RR;Collins,RA;Cole,MD;Lambowitz,AM;Heckman,JE;Yin,S;RajBhandary,UL
通讯作者：
RajBhandary,UL