THE T11-GLYCOPROTEIN IS FUNCTIONALLY LINKED TO A CALCIUM-CHANNEL IN PRECURSOR AND MATURE T-LINEAGE CELLS
THE T11-GLYCOPROTEIN IS FUNCTIONALLY LINKED TO A CALCIUM-CHANNEL IN PRECURSOR AND MATURE T-LINEAGE CELLS
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DOI：
10.1073/pnas.83.8.2614
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发表时间：
1986-04-01
影响因子：
11.1
通讯作者：
REINHERZ, EL
中科院分区：
文献类型：
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作者：
ALCOVER, A;WEISS, MJ;REINHERZ, EL
人类T淋巴细胞通过抗原/主要组织相容性复合体受体(T3-Ti)或T11绵羊红细胞结合蛋白被激活。用荧光分光光度法和多参数流式细胞仪技术研究激活与胞浆游离钙浓度变化的关系。结果发现，T_3-钛受体引起的细胞内[Ca~(2+)]i升高在很大程度上依赖于(Apprxeq)。80%)对细胞外钙离子的作用，程度小得多(.apprxeq.20%)对内部钙库的动员。此外，T11介导的[Ca~(2+)]i升高完全依赖于细胞外的Ca~(2+)。尽管抗T_3~(2+)和T_(11)的单抗诱导的[Ca~(2+)]i变化的动力学不同，但这两种途径在其他方面是相似的，特别是在对质膜电位的影响或由质膜电位介导的方面。重要的是，T11通路在缺乏表面T3-Ti复合体的前体T细胞中被发现具有功能。这些发现表明，可能存在一个功能或物理上与T11结构相连的质膜钙通道，或者，可能存在一组与T11和T3-Ti相关的钙通道。
Human T lymphocytes are activated through either the antigen/major histocompatibility complex receptor (T3-Ti) or the T11 sheep erythrocyte-binding protein. Spectrofluorimetry and multiparameter flow cytometric techniques were utilized to examine the relationship of activation to alterations in cytoplasmic free calcium concentration, [Ca2+]i. T3-Ti receptor-triggered elevation in [Ca2+]i was found to be dependent in large part (.apprxeq. 80%) on extracellular Ca2+ and to a much smaller extent (.apprxeq. 20%) on mobilization of internal Ca2+ pools. Furthermore, T11-mediated increases in [Ca2+]i were entirely dependent on extracellular Ca2+. Though the kinetics of [Ca2+]i changes induced by monoclonal antibodies to T3-Ti and T11 differed, both pathways were otherwise similar, particularly with regard to effects on or mediated by the plasma membrane potential. Importantly, the T11 pathway was found to be functional in precursor T-lineage cells lacking the surface T3-Ti complex. These findings suggest that there may be a plasma membrane Ca2+ channel functionally or physically linked to the T11 structure or, alternatively, that there is a set of related T11- and T3-Ti-associated Ca2+ channels.