Determination of medecamycin by adsorptive stripping voltammetry with an activated electrode
Determination of medecamycin by adsorptive stripping voltammetry with an activated electrode
复制标题
活化电极吸附溶出伏安法测定麦迪霉素
DOI：
10.1007/s00216-004-2866-9
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发表时间：
2004
影响因子：
4.3
通讯作者：
G. Chen
中科院分区：
文献类型：
--
作者：
N. Li;H. Luo;G. Chen
在0.025 mol l - 1NH3-NH4Cl （pH 8.6）溶液中，采用+1.8 V阳极氧化预处理玻璃碳电极（GCE），研究了medecamycin在+1.8 V阳极氧化5 min条件下的吸附和电化学行为。建立了吸附溶出伏安法在预处理过的玻碳电极上测定美地霉素的方法。在- 0.7 V（相对于饱和甘汞电极（SCE））电位下，将美地霉素在pH为9.0的NH3-NH4Cl缓冲液中积累一定时间，然后用二阶微分阳极溶出伏安法测定。+0.72 V的二阶微分阳极溶出峰电流与美地霉素浓度在2.0 ~ 50.0 μ ml−1范围内成正比。对10.0 μ ml - 1的美地霉素连续8次检测，检测限为1.0 μ ml - 1（信噪比为3倍），相对标准偏差为3.28%。该方法可用于直接测定市售片剂和加标尿样中美地霉素的含量，结果满意。
The adsorptive and electrochemical behaviors of medecamycin were investigated on a glassy carbon electrode (GCE) pretreated by anodic oxidation at +1.8 V for 5 min in 0.025 mol l−1NH3-NH4Cl (pH 8.6) solution. An adsorptive stripping voltammetric method for the determination of medecamycin at the pretreated glassy carbon electrode has been developed. Medecamycin was accumulated in NH3-NH4Cl buffer (pH 9.0) at a potential of −0.7 V (vs. saturated calomel electrode (SCE)) for a certain time, and then determined by second-order differential anodic stripping voltammetry. The second-order differential anodic stripping peak current at +0.72 V was proportional to the concentration of medecamycin in the range 2.0 μg ml−1to 50.0 μg ml−1. The detection limit (three times the signal-to-noise) was 1.0 μg ml−1and the relative standard deviation of the results was 3.28% for eight successive determinations of 10.0 μg ml−1medecamycin. This method has been applied to the direct determination of medecamycin in commercial tablets and spiked urine samples with satisfactory results.