Factors affecting success of embryo collection and transfer in large dairy herds
Factors affecting success of embryo collection and transfer in large dairy herds
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DOI：
10.1016/j.theriogenology.2007.09.008
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发表时间：
2008-01-01
期刊：
影响因子：
2.8
通讯作者：
Metzger, J.
中科院分区：
文献类型：
--
作者：
Chebel, R. C.;Demetrio, D. G. B.;Metzger, J.
我们的目的是评估影响大型奶牛群胚胎移植计划成功的因素。非泌乳供体奶牛的卵子/胚胎数（P < 0.01）和活胚数（P < 0.01）均高于泌乳奶牛。季节和供体类别之间的交互作用与受精卵/胚胎比例相关（P = 0.03），因为夏季哺乳期供体受精卵较少。305日龄等效产奶量与过度刺激反应无相关性。虽然超刺激方案之间的间隔与卵/胚数相关（P = 0.03），但与活胚数无关。小母牛受胎率与胚胎质量等级（P < 0.01）、季节（P = 0.04）、胚胎是新鲜还是冷冻/解冻相关（P < 0.01）。泌乳受体奶牛的P/ET率在夏季呈较低趋势（P = 0.12 ~ P = 0.08）。同步协议倾向于（P = 0.06; Herd 1）或（P = 0.02; Herd 2）与P/ET相关。接受玻璃化试管婴儿胚胎的奶牛P/ET低于接受新鲜试管婴儿胚胎的奶牛（P = 0.01），特别是在夏季（P = 0.09）。产奶量与P/ET不相关。使用散热系统对提高胚胎产量和P/ET至关重要。同步方案，优化同步排卵可能会增加受精卵奶牛的生育能力，并消除需要动情检测。(c) 2007年Elsevier Inc.出版
Our objective was to evaluate factors that affected the success of embryo transfer programs in large dairy herds. Non-lactating donor cows produced a larger number of ova/embryos (P < 0.01) and viable embryos (P < 0.01) than lactating cows. The interaction between season and donor class was correlated with the proportion of ova/embryos classified as fertilized (P = 0.03), because lactating donors had fewer fertilized ova in the summer. There was no correlation between 305-day mature equivalent milk yield and response to superstimulation. Although the interval between superstimulation protocols was correlated with the number of ova/embryos (P = 0.03), there was no correlation with the number of viable embryos. Pregnancy per embryo transfer (P/ET) in heifer recipients was correlated with embryo quality grade (P < 0.01), season (P = 0.04), and whether embryos were fresh or frozen/thawed (P < 0.01). Lactating recipient cows tended to have a lower rate of P/ET during the summer (P = 0.12 to P = 0.08). Synchronization protocols tended to be (P = 0.06; Herd 1) or were (P = 0.02; Herd 2) correlated with P/ET. Lactating cows receiving vitrified IVF embryos had a lower (P = 0.01) P/ET than those receiving fresh IVF embryos, especially in the summer (P = 0.09). Milk yield was not correlated with P/ET. The use of heat abatement systems is critical to improve embryo production and P/ET. Synchronization protocols that optimized synchrony of ovulation may increase fertility of recipient cows and eliminate the need for estrous detection. (c) 2007 Published by Elsevier Inc.