Angiostrongylus cantonensis: Identification and characterization of microRNAs in male and female adults
Angiostrongylus cantonensis: Identification and characterization of microRNAs in male and female adults
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DOI：
10.1016/j.exppara.2011.02.019
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发表时间：
2011-06-01
影响因子：
2.1
通讯作者：
Chen, Jia-Xu
中科院分区：
文献类型：
--
作者：
Chen, Mu-Xin;Ai, Lin;Chen, Jia-Xu
广州管圆线虫在人类中引起嗜酸性粒细胞性脑膜炎和嗜酸性粒细胞性白细胞增多，在全球具有重要的社会经济意义。microRNAs（miRNAs）是一类内源性小分子非编码RNA，在基因表达调控、细胞功能和防御、稳态和发病机制等方面发挥重要作用。它们已在多种生物体中被发现。本研究的目的是确定和表征雌性和雄性成虫的miRNA。通过Solexa deep sequencing对广州莲子草进行测序。在女性和男性中分别获得了总共8861，260和10，957，957个具有20和23个保守miRNA的高质量读段。核苷酸偏好性分析显示，尿嘧啶是主要的核苷酸，尤其是在第1、10、14、17和22位，大约在起始、中间和末端。据我们所知，这是第一份关于A. cantonensis的基因表达调控研究，可能为A.广东话。(C)2011 Elsevier Inc. All rights reserved.
Angiostrongylus cantonensis causes eosinophilic meningitis and eosinophilic pleocytosis in humans and is of significant socio-economic importance globally. microRNAs (miRNAs) are endogenous small non-coding RNAs that play crucial roles in gene expression regulation, cellular function and defense, homeostasis and pathogenesis. They have been identified in a diverse range of organisms. The objective of this study was to determine and characterize miRNAs of female and male adults of A. cantonensis by Solexa deep sequencing. A total of 8861,260 and 10,957,957 high quality reads with 20 and 23 conserved miRNAs were obtained in females and males, respectively. No new miRNA sequence was found. Nucleotide bias analysis showed that uracil was the prominent nucleotide, particularly at positions of 1, 10, 14, 17 and 22, approximately at the beginning, middle and the end of the conserved miRNAs. To our knowledge, this is the first report of miRNA profiles in A. cantonensis, which may represent a new platform for studying regulation of genes and their networks in A. cantonensis. (C) 2011 Elsevier Inc. All rights reserved.