Discovery of Missing Methylation Sites on Endogenous Peptides of Human Cell Lines
Discovery of Missing Methylation Sites on Endogenous Peptides of Human Cell Lines
复制标题
人类细胞系内源肽缺失甲基化位点的发现
DOI：
10.1007/s13361-019-02270-y
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发表时间：
2019-08
影响因子：
3.2
通讯作者：
Jia Chenxi
中科院分区：
文献类型：
--
作者：
Yan Xin;Li Lingjun;Jia Chenxi
Methylation of proteins has considerable impacts on physiological processes including signal transduction, DNA damage repair, transcriptional regulation, gene activation, and inhibition of gene expression. However, the traditional proteomics-based approach suffers from limited identification rates of these critical methylation sites on endogenous peptides. In this work, a peptidomics-based workflow was established to discover and characterize the global methylome of endogenous peptides in human cells. The reliability of our strategy was validated by methyl-SILAC labeling, resulting in 83% true-positive identifications in the HeLa cell line. We applied this approach to seven human cell lines, and 700 methylated forms on 646 putative methylation sites were identified in total, with over 61% of the methylation sites being newly identified. This study provides a complementary strategy for a traditional proteomics-based approach that enables identification of missing methylation sites and creates a first methylome draft of endogenous peptides of human cell lines, offering a valuable resource for in-depth studies of biological functions of methylated endogenous peptides.
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影响因子：
4.4
作者：
Gelman, Julia S.;Sironi, Juan;Castro, Leandro M.;Ferro, Emer S.;Fricker, Lloyd D.
通讯作者：
Fricker, Lloyd D.
DOI：
10.1007/978-1-4939-7537-2_17
发表时间：
2018-01-01
期刊：
PEPTIDOMICS: METHODS AND STRATEGIES
影响因子：
--
作者：
DeLaney, Kellen;Buchberger, Amanda;Li, Lingjun
通讯作者：
Li, Lingjun
蛋白质组分析前样品制备的蛋白质沉淀方法的比较。
影响因子：
4.1
作者：
Jiang, L;He, L;Fountoulakis, M
通讯作者：
Fountoulakis, M
影响因子：
7.4
作者：
Jung, Sung Yun;Li, Yehua;Qin, Jun
通讯作者：
Qin, Jun
影响因子：
4.8
作者：
Garcia, Benjamin A.;Hake, Sandra B.;Hunt, Donald F.
通讯作者：
Hunt, Donald F.