Reassessing the role of defensin in the innate immune response of the mosquito, Aedes aegypti
Reassessing the role of defensin in the innate immune response of the mosquito, Aedes aegypti
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DOI：
10.1111/j.0962-1075.2004.00467.x
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发表时间：
2004-04-01
影响因子：
2.6
通讯作者：
Christensen, BM
中科院分区：
文献类型：
--
作者：
Bartholomay, LC;Fuchs, JF;Christensen, BM
防御素是埃及伊蚊体内主要的诱导性免疫肽。尽管其在体外对革兰氏阳性细菌具有活性，但在接种革兰氏阳性或阴性细菌或蠕虫的蚊子中检测到防御素表达。防御素的转录和表达依赖于细菌剂量;然而，翻译与转录不一致，因为肽仅在接种大剂量的蚊子中可检测到。体外翻译试验为防御素的转录后调节提供了进一步的证据。清除试验表明，大多数细菌在检测到防御素之前被清除。在基因沉默实验中，在接种细菌后，防御素缺陷型和对照蚊子之间没有观察到死亡率的显著差异。这些研究表明，防御素可能在蚊子免疫中具有替代功能。
Defensin is the predominant inducible immune peptide in Aedes aegypti. In spite of its activity against Gram-positive bacteria in vitro, defensin expression is detected in mosquitoes inoculated with Gram-positive or negative bacteria, or with filarial worms. Defensin transcription and expression are dependent upon bacterial dose; however, translation is inconsistent with transcription because peptide is detectable only in mosquitoes inoculated with large doses. In vitro translation assays provide further evidence for post-transcriptional regulation of defensin. Clearance assays show that a majority of bacteria are cleared before defensin is detected. In gene silencing experiments, no significant difference in mortality was observed between defensin-deficient and control mosquitoes after bacteria inoculation. These studies suggest that defensin may have an alternative function in mosquito immunity.