High-throughput detection of DNA double-strand breaks using image cytometry.

High-throughput detection of DNA double-strand breaks using image cytometry.
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DOI:
10.2144/000114248
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发表时间:
2015-01
期刊:
影响因子:
2.7
通讯作者:
Kimple RJ
Kimple RJ
中科院分区:
工程技术4区
文献类型:
--
作者:
Fowler TL;Bailey AM;Bednarz BP;Kimple RJ

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Assessment of γH2AX expression for studying DNA double-strand break formation is often performed by manual counting of foci using immunofluorescence microscopy, an approach that is laborious and subject to significant foci selection bias. Here we present a novel high-throughput method for detecting DNA double-strand breaks using automated image cytometry assessment of cell average γH2AX immunofluorescence. Our technique provides an expedient, high-throughput, objective, and cost-effective method for γH2AX analysis.
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DOI: 10.1007/978-1-61779-998-3_40
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