High-throughput detection of DNA double-strand breaks using image cytometry.
High-throughput detection of DNA double-strand breaks using image cytometry.
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DOI:
10.2144/000114248
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发表时间:
2015-01
期刊:
影响因子:
2.7
通讯作者:
Kimple RJ
中科院分区:
文献类型:
--
作者:
Fowler TL;Bailey AM;Bednarz BP;Kimple RJ
Assessment of γH2AX expression for studying DNA double-strand break formation is often performed by manual counting of foci using immunofluorescence microscopy, an approach that is laborious and subject to significant foci selection bias. Here we present a novel high-throughput method for detecting DNA double-strand breaks using automated image cytometry assessment of cell average γH2AX immunofluorescence. Our technique provides an expedient, high-throughput, objective, and cost-effective method for γH2AX analysis.
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影响因子:
3.5
作者:
Fowler, Tyler L.;Fulkerson, Regina K.;Bednarz, Bryan P.
通讯作者:
Bednarz, Bryan P.
DOI:
10.1016/j.mrfmmm.2013.07.007
发表时间:
2013-10
影响因子:
2.3
作者:
Scully, Ralph;Xie, Anyong
通讯作者:
Xie, Anyong
影响因子:
3.7
作者:
Schuler N;Palm J;Kaiser M;Betten D;Furtwängler R;Rübe C;Graf N;Rübe CE
通讯作者:
Rübe CE
DOI:
10.1007/978-1-61779-998-3_40
发表时间:
2012-01-01
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
--
作者:
Sharma, Arishya;Singh, Kamini;Almasan, Alexandru
通讯作者:
Almasan, Alexandru