EXPERIMENTAL DUCK HEPATITIS-B VIRUS-INFECTION - PATHOLOGY AND EVOLUTION OF HEPATIC AND EXTRAHEPATIC INFECTION
EXPERIMENTAL DUCK HEPATITIS-B VIRUS-INFECTION - PATHOLOGY AND EVOLUTION OF HEPATIC AND EXTRAHEPATIC INFECTION
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DOI：
10.1002/hep.1840080313
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发表时间：
1988-05-01
期刊：
影响因子：
13.5
通讯作者：
COSSART, YE
中科院分区：
文献类型：
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作者：
FREIMAN, JS;JILBERT, AR;COSSART, YE
对70只1日龄腹腔接种鸭乙型肝炎病毒的雏鸭和30只对照雏鸭进行了为期2年的研究。所有接种的鸭子都感染了鸭乙型肝炎病毒，但这与临床发病无关。采用斑点杂交、Southern印迹杂交和原位杂交技术，在第3天于肝脏、第4天于胰腺腺泡细胞、第6天于血清、第7天于脾脏红髓和白髓以及第14天于肾小球中首次检测到鸭乙型肝炎病毒DNA。通过DNA聚合酶水平测定，循环病毒的峰值出现在接种后1至4周。在感染和对照鸭子的肝组织切片中均观察到轻度的门脉炎症。然而，22只感染鸭中有8只出现中度严重的炎症变化，而18只对照鸭中为0只（p < 0.025）。接种后6周出现这种炎症浸润，同时肝细胞和胰腺腺泡细胞内鸭乙型肝炎病毒DNA水平下降。与此同时，鸭乙型肝炎病毒DNA越来越多地局限于脾脏生发中心，并且首次在胰岛细胞中检测到病毒DNA。肝外组织感染未伴有组织学变化。结合人类乙型肝炎病毒感染的发病机制，讨论了鸭乙型肝炎病毒在肝脏和肝外组织中感染的顺序和意义。
Seventy, 1-day-old ducklings inoculated intraperitoneally with duck hepatitis B virus and 30 controls have been studied over a 2-year period. Infection with duck hepatitis B virus occurred in all inoculated ducks, although this was not associated with clinical morbidity. Duck hepatitis B virus DNA was first detected in liver on Day 3, in pancreatic acinar cells on Day 4, serum on Day 6, splenic red and white pulp on Day 7 and in the renal glomurulus on Day 14, using a combination of dot, Southern blot and in situ hybridization techniques. Peak levels of circulating virus, as determined by DNA polymerase levels, occurred 1 to 4 weeks postinoculation. Mild degrees of portal inflammation were seen in sections of liver tissue in both infected and control ducks. However, moderately severe inflammatory changes were present in 8 of 22 infected birds compared with 0 of 18 controls (p < 0.025). Appearance of this inflammatory infiltrate 6 weeks postinocultion coincided with a decrease in levels of duck hepatitis B virus DNA in hepatocytes and within the pancreatic acinar cells. At the same time, duck hepatitis B virus DNA became increasingly localized to the splenic germinal centers, and viral DNA was first detected in pancreatic islet cells. No histological changes accompanied the extrahepatic tissue infection. The sequence and significance of duck hepatitis B virus infection in liver and extrahepatic tissues is discussed in relation to the pathogenesis of hepatitis B virus infection in man.