Apical complex genes control mitotic spindle geometry and relative size of daughter cells in Drosophila neuroblast and pl asymmetric divisions
Apical complex genes control mitotic spindle geometry and relative size of daughter cells in Drosophila neuroblast and pl asymmetric divisions
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DOI：
10.1016/s0092-8674(02)01170-4
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发表时间：
2003-01-10
期刊：
影响因子：
64.5
通讯作者：
Yang, XH
中科院分区：
文献类型：
--
作者：
Cai, Y;Yu, FW;Yang, XH
果蝇神经母细胞的不对称分裂产生了两个大小和命运不同的女儿。顶端定位的分子复合体介导了细胞命运决定因素的基本定位和有丝分裂纺锤体的顶端定位，但子代细胞的大小是如何控制的仍不清楚。在这里，我们发现有丝分裂纺锤体的几何形状和不等的子细胞大小是由顶端复合体内的两条平行通路(Bazooka/DaPKC和Pins/Galphai)控制的。虽然两条途径中的任何一条单独的局部活性足以调节不对称有丝分裂纺锤体和大小不等的神经母细胞子代的产生，但这两条途径的缺失会导致对称分裂。在感觉器官前体中，Bazooka/DaPKC和Pins/Galphai位于大脑皮层的对侧，并以相反的方式发挥作用，产生对称的纺锤体。
Drosophila neuroblast asymmetric divisions generate two daughters of unequal size and fate. A complex of apically localized molecules mediates basal localization of cell fate determinants and apicobasal orientation of the mitotic spindle, but how daughter cell size is controlled remains unclear. Here we show that mitotic spindle geometry and unequal daughter cell size are controlled by two parallel pathways (Bazooka/DaPKC and Pins/Galphai) within the apical complex. While the localized activity of either pathway alone is sufficient to mediate the generation of an asymmetric mitotic spindle and unequal size neuroblast daughters, loss of both pathways results in symmetric divisions. In sensory organ precursors, Bazooka/DaPKC and Pins/Galphai localize to opposite sides of the cortex and function in opposition to generate a symmetric spindle.