Effects of peripheral administration of lipopolysaccharide on chronic sickness responses in TRPM8-deficient mice.
Effects of peripheral administration of lipopolysaccharide on chronic sickness responses in TRPM8-deficient mice.
复制标题
脂多糖外周给药对 TRPM8 缺陷小鼠慢性病反应的影响。
DOI：
10.1016/j.neulet.2022.136895
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发表时间：
2022
影响因子：
2.5
通讯作者：
Miyata S.
中科院分区：
文献类型：
--
作者：
Horikawa R;Oe Y;Fujii R;Kasuga K;Yoshimura R;Miyata S.
瞬时受体电位melastatin 8 (TRPM8)是冷感温度感受器阳离子通道；然而，其在内毒素诱导的神经炎症中的功能作用仍不清楚。在本研究中，我们研究了 TRPM8 敲除 (KO) 小鼠在脂多糖 (LPS) 诱导的败血症期间的慢性疾病反应。与野生型 (WT) 小鼠相比，腹腔内给予 5 mg/kg LPS 在 TRPM8 KO 小鼠中产生更持久的低温。与 WT 小鼠相比，TRPM8 KO 小鼠在 LPS 给药后还表现出比 WT 小鼠更持久的运动活动、体重以及食物和水摄入量下降。此外，LPS诱导的白细胞和淋巴细胞数量减少在TRPM8 KO小鼠中持续的时间比在WT小鼠中持续的时间更长。目前的结果表明 TRPM8 减弱了内毒素诱导的脓毒症的慢性疾病反应。
Transient receptor potential melastatin 8 (TRPM8) is a cold-sensing thermoreceptor cation channel; however, its functional role in endotoxin-induced neuroinflammation remains unclear. In the present study, we investigated chronic sickness responses in TRPM8 knockout (KO) mice during lipopolysaccharide (LPS)-induced sepsis. The intraperitoneal administration of 5 mg/kg LPS generated longer-lasting hypothermia in TRPM8 KO mice than in wild-type (WT) mice. TRPM8 KO mice also exhibited longer-lasting declines in locomotor activity, body weight, and food and water intakes than WT mice upon LPS administration. In addition, LPS-induced decreases in the numbers of leucocytes and lymphocytes that persisted for a longer time in TRPM8 KO mice than in WT mice. The present results indicate TRPM8 attenuated chronic sickness responses in endotoxin-induced sepsis.