Three-Dimensional Printed Polylactic Acid Scaffolds Promote Bone-like Matrix Deposition in Vitro
Three-Dimensional Printed Polylactic Acid Scaffolds Promote Bone-like Matrix Deposition in Vitro
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DOI：
10.1021/acsami.9b02502
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发表时间：
2019-05-01
影响因子：
9.5
通讯作者：
Haglund, Lisbet
中科院分区：
文献类型：
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作者：
Fairag, Rayan;Rosenzweig, Derek H.;Haglund, Lisbet
大面积骨缺损是临床医生和外科医生面临的重大挑战。骨再生的组织工程代表了这一困境的创新解决方案，并可能产生有吸引力的替代骨替代品。使用廉价的桌面打印机进行三维（3D）打印显示出使用生物相容性，生物可降解性和成本效益的热塑性塑料生成模拟天然组织的高分辨率结构的前景，这些热塑性塑料已经被FDA批准用于食品，药物输送和许多医疗设备。微孔3D打印的聚乳酸支架，具有不同的孔径（500，750和1000 μ m），设计和制造使用廉价的台式3D打印机，并评估机械性能。使用原代人成骨细胞比较支架的细胞生长、活性和骨样组织形成。成骨细胞表现出高增殖，代谢活性，和成骨基质蛋白的生产，其中750 μ m孔径的支架显示出优越性。在750 μ m孔隙支架上使用人间充质干细胞的进一步实验显示了它们支持成骨分化的能力。这些发现表明，即使在没有任何表面修饰的情况下，低成本的750 μ m孔径的3D打印支架也可能适合作为修复大骨缺损的骨替代品。
Large bone defects represent a significant challenge for clinicians and surgeons. Tissue engineering for bone regeneration represents an innovative solution for this dilemma and may yield attractive alternate bone substitutes. Three-dimensional (3D) printing with inexpensive desktop printers shows promise in generating high-resolution structures mimicking native tissues using biocompatible, biodegradable, and cost-effective thermoplastics, which are already FDA-approved for food use, drug delivery, and many medical devices. Microporous 3D-printed polylactic acid scaffolds, with different pore sizes (500, 750, and 1000 mu m), were designed and manufactured using an inexpensive desktop 3D printer, and the mechanical properties were assessed. The scaffolds were compared for cell growth, activity, and bone-like tissue formation using primary human osteoblasts. Osteoblasts showed high proliferation, metabolic activity, and osteogenic matrix protein production, in which 750 mu m pore-size scaffolds showed superiority. Further experimentation using human mesenchymal stem cells on 750 mu m pore scaffolds showed their ability in supporting osteogenic differentiation. These findings suggest that even in the absence of any surface modifications, low-cost 750 mu m pore-size 3D-printed scaffolds may be suitable as a bone substitute for repair of large bone defects.