Gadodiamide T1 relaxivity in brain tissue in vivo is lower than in saline.
Gadodiamide T1 relaxivity in brain tissue in vivo is lower than in saline.
复制标题
体内脑组织中的钆双酰胺 T1 弛豫度低于盐水中。
DOI：
10.1002/mrm.20336
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发表时间：
2005
影响因子：
3.3
通讯作者：
Kundel,HaroldL
中科院分区：
文献类型：
--
作者：
Pickup,Stephen;Wood,AndrewKW;Kundel,HaroldL
在受伤和正常大鼠大脑中，在 4.7 T 下对钆双酰胺 (Gd-DTPA-BMA)T1 松弛度进行体内测量。通过局部冷冻法在 9 只大鼠中诱导脑损伤。在注射 Gd-DTPA-BMA (0.1-0.6 mmol/kg) 之前和之后生成损伤的 T1 图。死后收集正常和坏死大脑的样本；测定湿重和干重，并通过电感耦合等离子体质谱法测量Gd含量。体内弛豫率通过注射造影剂后弛豫率变化图的线性拟合确定，作为Gd含量的函数。该分析得出受伤大脑在 36°C 下的弛豫度为 2.8 秒−1mmol−1kg 组织水。受伤大脑中的水重量分数为 0.90 ± SD 0.02 wt/wt，正常大脑中的水重量分数为 0.79 ± 0.02。还在室温和生理温度下对生理盐水中的 Gd-DTPA-BMA (0.0–0.6 mmol) 和白蛋白 (0–30% wt/wt) 溶液进行了弛豫测量。白蛋白/盐水的弛豫度随着固体含量的增加而增加，21°C 时值为 4.0–4.9 sec−1mmol−1kg，37°C 时值为 3.4–4.5 sec−1mmol−1kg。组织的弛豫率与固体含量相当的盐水溶液显着不同，这表明组织的固体含量并不是决定体内弛豫率的唯一因素。 Magn Reson Med 53:35–40, 2005。© 2004 Wiley‐Liss, Inc.
In vivo measurements of gadodiamide (Gd‐DTPA‐BMA)T1relaxivity were performed at 4.7 T in injured and normal rat brains. Cerebral lesions were induced in nine rats by a localized freezing method.T1maps of the lesions were generated before and after injection of Gd‐DTPA‐BMA (0.1–0.6 mmol/kg). Samples of normal and necrotic brain were collected postmortem; the wet and dry weights were determined, and Gd content was measured by inductively coupled plasma mass spectroscopy. The in vivo relaxivity was determined by a linear fit of a plot of the change in relaxation rate following injection of the contrast agent as a function of Gd content. This analysis yielded a relaxivity in the injured brain of 2.8 sec−1mmol−1kg tissue water at 36°C. The water weight fraction was 0.90 ± SD 0.02 wt/wt in injured brain and 0.79 ± 0.02 in normal brain. Relaxivity measurements were also performed on solutions of Gd‐DTPA‐BMA (0.0–0.6 mmol) and albumin (0–30% wt/wt) in normal saline at room and physiologic temperatures. The relaxivity in the albumin/saline increased with increasing solids content with values of 4.0–4.9 sec−1mmol−1kg at 21°C and 3.4–4.5 sec−1mmol−1kg at 37°C. The relaxivity of the tissues differed significantly from that of the saline solutions of comparable solids content, suggesting that the solids content of a tissue is not the only factor that determines in vivo relaxivity. Magn Reson Med 53:35–40, 2005. © 2004 Wiley‐Liss, Inc.