Expression patterns and functional analysis of the short neuropeptide F and NPF receptor genes in Rhopalosiphum padi
Expression patterns and functional analysis of the short neuropeptide F and NPF receptor genes in Rhopalosiphum padi
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指地丝虫短神经肽F和NPF受体基因的表达模式及功能分析
DOI：
10.1111/1744-7917.12842
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发表时间：
2021
期刊：
影响因子：
4
通讯作者：
Maohua Chen
中科院分区：
文献类型：
--
作者：
Xiong Peng;Cheng Chen;Yixiao Huang;Suji Wang;Sha Su;Maohua Chen
短神经肽F（sNPF）和NPF受体（NPFR）基因在许多生理过程中起重要作用。然而，在不同的胁迫条件下，蚜虫的sNPF和NPFRs的生存相关功能的信息是缺乏的。在这项研究中，我们克隆了NPF和NPFR基因，并研究了这些基因在不同发育阶段、翅型和胁迫条件下的表达水平，一种重要的农业害虫NPF和NPFRs的转录水平在不同发育阶段有差异，有翅雌虫的表达水平显著高于无翅雌虫。此外，饥饿导致显著增加的sNPF表达，然后恢复后再喂养。热胁迫和杀虫剂显著影响了这两个基因的转录。padiusing RNA干扰在注射后48 h显示最佳干扰效率。sNPF敲低显著降低成虫寿命、15 d繁殖力和食物摄入量。此外，在注射双链NPFinR后，饥饿、杀虫剂和热应激条件下的死亡率显著更高。NPFRs基因敲低显著影响水稻的摄食量和饥饿抗性。帕迪。这些结果有力地表明，sNPF在R的食物摄取、寿命和繁殖中起着重要作用。padi，并且它可以显著影响害虫对胁迫条件的反应。
The short neuropeptide F (sNPF) and NPF receptor (NPFR) genes play important roles in many physiological processes. However, information on the survival‐related functions ofsNPFandNPFRunder different stress conditions is lacking in aphids. In this study, we clonedsNPFandNPFR, and investigated the expression levels of these genes in different developmental stages, wing morphs, and stress conditions of the bird cherry‐oat aphid (Rhopalosiphum padiL.), an important agricultural pest. ThesNPFandNPFRtranscript levels varied among developmental stages, and their expression levels in alate females were significantly higher than those in apterous females. In addition, starvation resulted in significantly increased sNPFexpression, which then recovered after refeeding. Heat stress and insecticides significantly affected transcription of both genes.sNPFandNPFRknockdown inR. padiusing RNA interference revealed optimal interference efficiency at 48 h post‐injection.sNPFknockdown significantly decreased adult longevity, 15‐d fecundity, and food intake. Additionally, mortality under starvation, insecticides, and heat stress conditions was significantly higher after injection with double‐strandedsNPFinR. padi.NPFRknockdown significantly affected food intake and starvation resistance inR. padi. These results strongly indicate thatsNPFplays vital roles in food intake, longevity, and reproduction inR. padi, and it can significantly affect the pest's response to stress conditions.