Comparison of Nasal and Bronchial Epithelial Cells Obtained from Patients with COPD
Comparison of Nasal and Bronchial Epithelial Cells Obtained from Patients with COPD
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DOI：
10.1371/journal.pone.0032924
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发表时间：
2012-03-06
期刊：
影响因子：
3.7
通讯作者：
Ennis, Madeleine
中科院分区：
文献类型：
--
作者：
Comer, David M.;Elborn, J. Stuart;Ennis, Madeleine
对于气道病理生理的体外研究，原代上皮细胞比永生化细胞系具有许多优势。鼻上皮细胞比支气管上皮细胞更容易获得，可以作为体外研究的替代方法。我们的目的是比较COPD患者的鼻腔和支气管上皮细胞，以确定这些细胞是否对促炎刺激有相似的反应。在铜绿假单胞菌脂多糖刺激之前，将21名受试者配对鼻腔和支气管刷的细胞培养物与香烟烟雾提取物（CSE）孵育。ELISA法检测IL-6和IL-8， RT-PCR和FACS法检测toll样受体4 （TLR-4）的信息和表达。IL-8释放在两种细胞类型之间有显著相关性。与鼻上皮细胞相比，支气管细胞分泌IL-6明显减少，分泌浓度不相关。CSE孵育4小时对鼻腔和支气管细胞均有免疫抑制作用，而孵育24小时仅对鼻腔细胞有促炎作用。CSE仅在24 h后降低支气管细胞中TLR-4的表达，对mRNA表达无影响。在COPD患者中，鼻上皮细胞在气道炎症研究中不能代替体外支气管上皮细胞。
For in vitro studies of airway pathophysiology, primary epithelial cells have many advantages over immortalised cell lines. Nasal epithelial cells are easier to obtain than bronchial epithelial cells and can be used as an alternative for in vitro studies. Our objective was to compare nasal and bronchial epithelial cells from subjects with COPD to establish if these cells respond similarly to pro-inflammatory stimuli. Cell cultures from paired nasal and bronchial brushings (21 subjects) were incubated with cigarette smoke extract (CSE) prior to stimulation with Pseudomonas aeruginosa lipopolysaccharide. IL-6 and IL-8 were measured by ELISA and Toll-like receptor 4 (TLR-4) message and expression by RT-PCR and FACS respectively. IL-8 release correlated significantly between the two cell types. IL-6 secretion was significantly less from bronchial compared to nasal epithelial cells and secreted concentrations did not correlate. A 4 h CSE incubation was immunosuppressive for both nasal and bronchial cells, however prolonged incubation for 24 h was pro-inflammatory solely for the nasal cells. CSE reduced TLR-4 expression in bronchial cells only after 24 h, and was without effect on mRNA expression. In subjects with COPD, nasal epithelial cells cannot substitute for in vitro bronchial epithelial cells in airway inflammation studies.